T6455

Tris-HCl Buffer, pH 10, 10×, Antigen Retriever

• UNSPSC 코드: 12352107
• NACRES: NA.47

속성

• 생물학적 소스: synthetic
• Quality Level: 100
• pH: 10
• 응용 분야: hematology; histology
• 저장 온도: 2-8°C

설명

애플리케이션

Tris-HCl Buffer, pH 10, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Tris-HCl buffer, pH 10, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.

제조 메모

Dilute the Tris-HCl Buffer, pH 10, 10×, and Antigen Retriever 10-fold with water to prepare a 1 × Working Solution, e.g., dilute 10 mL of 10 × concentrate with 90 mL of water.

저장 및 안정성

Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.

기타 정보

Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer. Store the prepared 1′ Tris-HCl Buffer, pH 10, Working Solution at 2–8 °C.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable