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05-168

Sigma-Aldrich

Anti-TNFα Antibody, clone MPG-XT3

clone MPG-XT3, Upstate®, from rat

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MPG-XT3, monoclonal

species reactivity

mouse

manufacturer/tradename

Upstate®

technique(s)

neutralization: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TNF(7124)

General description

The concentration of antibody needed to neutralize the bioactivity of TNF-α will depend on the cell type, the incubation conditions, and the biological response studied.

Specificity

mouse TNF-α; does not recognize human or rat TNF-α

Immunogen

Recombinant murine TNF-α

Application

Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
This Anti-TNFalpha Antibody, clone MPG-XT3 is validated for use in NEUT for the detection of TNFα.

Quality

routinely used to neutralize 50% of the cytotoxic effect of recombinant mouse TNF-α on actinomycin D treated L929 cells.

Target description

17 kDa

Linkage

Replaces: 04-1114

Physical form

0.01M Tris-glycine, 0.15M NaCl, pH 7.4
DEAE chromatography
Format: Purified

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J A Guevara Patiño et al.
Journal of immunology (Baltimore, Md. : 1950), 164(4), 1689-1694 (2000-02-05)
Apoptosis is one of the key regulatory mechanisms in tissue modeling and development. In the thymus, 95-98% of all thymocytes die by apoptosis because they failed to express a TCR with an optimal affinity for the selecting intrathymic peptide-MHC complexes.
A Kunitz-type protease inhibitor, bikunin, inhibits ovarian cancer cell invasion by blocking the calcium-dependent transforming growth factor-beta 1 signaling cascade
Kobayashi, H., et al
The Journal of Biological Chemistry, 278, 7790-7799 (2003)
Cytokine mRNA in the central nervous system of SCID mice infected with Toxoplasma gondii: importance of T-cell-independent regulation of resistance to T. gondii.
Hunter, C A, et al.
Infection and Immunity, 61, 4038-4044 (1993)
Malgorzata Romanowska et al.
PloS one, 5(3), e9701-e9701 (2010-03-20)
Psoriasis is one of the most frequent skin diseases world-wide. The disease impacts enormously on affected patients and poses a huge financial burden on health care providers. Several lines of evidence suggest that the nuclear hormone receptor peroxisome proliferator activator
C K Yu et al.
International archives of allergy and immunology, 112(1), 73-82 (1997-01-01)
Dermatophagoides farinae (Der f) is one of the most common species of dust mites that induce asthma and allergic rhinitis. We have reported that Der f challenge on sensitized mice elicited a distinct type of hypersensitivity, called early-type hypersensitivity (ETH)

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