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Anti-GRB2 Antibody, clone 3F2

clone 3F2, Upstate®, from mouse

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Synonym(s):
Anti-ASH, Anti-EGFRBP-GRB2, Anti-Grb3-3, Anti-MST084, Anti-MSTP084, Anti-NCKAP2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

3F2, monoclonal

species reactivity

bovine, mouse, hamster, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GRB2(2885)

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MAB3070SAB4501290SAB2500491
antibody form

purified antibody

antibody form

culture supernatant

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

3F2, monoclonal

clone

GrB-7, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

bovine, mouse, hamster, rat, human

species reactivity

human

species reactivity

rat, human, mouse

species reactivity

mouse, rat, human, pig

manufacturer/tradename

Upstate®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: 1:40000, western blot: 1:500-1:1000

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

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Specificity

N-terminal SH3 region of GRB2

Immunogen

Recombinant GRB2/Ash

Application

This Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2.

Quality

routinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract

Target description

23-25kDa

Physical form

Format: Purified
PBS containing 50% glycerol

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ramya Ganesan et al.
Journal of leukocyte biology, 103(5), 867-883 (2018-04-16)
The uptake of cholesterol carried by low-density lipoprotein (LDL) is tightly controlled in the body. Macrophages are not well suited to counteract the cellular consequences of excess cholesterol leading to their transformation into "foam cells," an early step in vascular
Protein-tyrosine phosphatase 1D modulates its own state of tyrosine phosphorylation
Stein-Gerlach, M., et al
The Journal of Biological Chemistry, 270, 24635-24637 (1995)
c-Cbl is transiently tyrosine-phosphorylated, ubiquitinated, and membrane-targeted following CSF-1 stimulation of macrophages
Wang, Y., et al
The Journal of Biological Chemistry, 271, 17-20 (1996)
M Rozakis-Adcock et al.
Nature, 363(6424), 83-85 (1993-05-06)
Many tyrosine kinases, including the receptors for hormones such as epidermal growth factor (EGF), nerve growth factor and insulin, transmit intracellular signals through Ras proteins. Ligand binding to such receptors stimulates Ras guanine-nucleotide-exchange activity and increases the level of GTP-bound
M Shakibaei et al.
The Biochemical journal, 342 Pt 3, 615-623 (1999-09-08)
We have examined the mechanism by which collagen-binding integrins co-operate with insulin-like growth factor-I (IGF-I) receptors (IGF-IR) to regulate chondrocyte phenotype and differentiation. Adhesion of chondrocytes to anti-beta1 integrin antibodies or collagen type II leads to phosphorylation of cytoskeletal and

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