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05-904

Sigma-Aldrich

Anti-HA Tag Antibody

Upstate®, from mouse

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eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

species reactivity

vertebrates (common species)

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG3

shipped in

dry ice

target post-translational modification

unmodified

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This Item
04-902GW22511SAB4300603
vibrant-m

05-904

Anti-HA Tag Antibody

clone

monoclonal

clone

DW2, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

vertebrates (common species)

species reactivity

vertebrates (common species)

species reactivity

-

species reactivity

-

antibody form

purified immunoglobulin

antibody form

culture supernatant

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

isotype

IgG3

isotype

IgG

isotype

-

isotype

IgG

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable

technique(s)

western blot: suitable

technique(s)

indirect ELISA: suitable

technique(s)

western blot: 1:500-1:1000

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General description

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Specificity

Reactivity with other species has not been confirmed.
Recognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 98-106 of influenza hemaglutinin.

Application

Detect HA Tag using this Anti-HA Tag Antibody validated for use in IC, IP & WB.
Immunoprecipitation:
5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.

Immunocytochemistry:
5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

Routinely evaluated by western blot of recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.

Target description

Various

Linkage

Replaces: 07-221

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal IgG3 in buffer containing PBS, pH 7.4 containing 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
RIPA lysates from HEK293 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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A rapid and efficient immunoenzymatic assay to detect receptor protein interactions: G protein-coupled receptors.
Zappelli, E; Daniele, S; Abbracchio, MP; Martini, C; Trincavelli, ML
International Journal of Molecular Sciences null
Verena Supper et al.
Journal of immunology (Baltimore, Md. : 1950), 196(3), 1387-1399 (2016-01-06)
The Ig superfamily member CD147 is upregulated following T cell activation and was shown to serve as a negative regulator of T cell proliferation. Thus, Abs targeting CD147 are being tested as new treatment strategies for cancer and autoimmune diseases.
H Wu et al.
Neuroscience, 170(1), 149-155 (2010-07-06)
Previous studies have demonstrated that merlin acts as a tumor suppressor by blocking Ras-mediated signaling. However, the mechanism by which merlin controls cell proliferation has remained obscure. Here we show that merlin deficient tumors exhibited loss of p21, concomitant with
Patrick Guye et al.
Nature communications, 7, 10243-10243 (2016-01-07)
Human induced pluripotent stem cells (hiPSCs) have potential for personalized and regenerative medicine. While most of the methods using these cells have focused on deriving homogenous populations of specialized cells, there has been modest success in producing hiPSC-derived organotypic tissues
Neutrophils activate alveolar macrophages by producing caspase-6-mediated cleavage of IL-1 receptor-associated kinase-M.
Kobayashi, H; Nolan, A; Naveed, B; Hoshino, Y; Segal, LN; Fujita, Y; Rom, WN; Weiden, MD
Journal of immunology (Baltimore, Md. : 1950) (1950)

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