06-863
Anti-CREB Antibody
Upstate®, from rabbit
Synonym(s):
Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1, CREB-1
About This Item
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
using Protein A
species reactivity
mouse, human, rat
species reactivity (predicted by homology)
porcine (based on 100% sequence homology), horse (based on 100% sequence homology), canine (based on 100% sequence homology)
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
electrophoretic mobility shift assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CREB1(1385)
General description
Specificity
Immunogen
Application
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected an enhanced CREB occupancy at the LYPD3 promoter in LKB1-deficient TE-4 human esophageal cancer cells (Gu, Y., et al. (2012). Oncogene. 31(4):469–479).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB association at the RNASET2 sites targeted by T-cell Leukemia Virus type 1 (HTLV-1) encoded Tax protein using HTLV-1-infected human T-cell lines, MT-2, C8166/45 and SLB-1 (Polakowski, N., et al. (2011). Viruses. 3(8):1485-500).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB occupancy at the hBDNF promoter IV using postmortem human parietal cortex tissue chromatin preparations (Pruunsild, P., et al. (2011). J. Neurosci. 31(9):3295-3308).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB occupancy at the miR-9-2 promoter in SK-N-BE human neuroblastoma cells (Laneve, P., et al. (2010). Nucl. Acids Res. 38(20):6895-6905).
ChIP-seq Analysis: A representative lot identified CREB target genes in murine spermatogonia derived GC1-spg cells by ChIP-seq analysis (Martianov, I., et al. (2010). BMC Genomics. 11:530).
Western Blotting Analysis: Representative lots detected CREB in lysates from cultured embryonic rat striatal neurons and PC12 pheochromocytoma cells (Hutchinson, A.N., et al. (2012). Neuropsychopharmacology. 37(2):321-337; Maharjan, S., et al. (2010). J. Neurochem. 112(1):42-55).
Western Blotting Analysis: Representative lots detected CREB in mouse proximal caput epididymis-1 (PC-1) cell lysate and hippocampus tissue homogenates (Hamzeh, M., and Robaire, B. (2011). J. Endocrinol. 209(1):55-64; Zhou, Z., et al. (2009). Neuropharmacology. 56(1):81-89).
Western Blotting Analysis: A representative lot detected CREB in SK-N-BE human neuroblastoma cell lysate (Laneve, P., et al. (2010). Nucl. Acids Res. 38(20):6895-6905).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-CREB complex in EMSA using radiolabeled oligonucleotide containing hBDNF promoter I CREB-binding sequence and lysates from KCl-treated primary rat neurons (Pruunsild, P., et al. (2011). J. Neurosci. 31(9):3295-3308).
Epigenetics & Nuclear Function
Transcription Factors
Quality
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CREB in 10 µg of HeLa nuclear extract.
Target description
Linkage
Physical form
Storage and Stability
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Other Notes
Legal Information
Disclaimer
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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