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GranuCult® Half FRASER broth (base) with antibiotics

pkg of 500 g, according to ISO 11290, for Listeria spp., suitable for pathogen testing

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Synonym(s):
Demi FRASER broth (base) with antibiotics

agency

APHA
according to ISO 11290

Quality Level

form

medium granules (dehydrated (DCM))

packaging

pkg of 500 g

manufacturer/tradename

GranuCult®

storage condition

protect from light (both UV light and sun light)

technique(s)

microbiological culture: suitable

pH

7.0-7.4 (25 °C, 55 g/L in H2O, after autoclaving)

solubility

55 g/L

application(s)

food and beverages
microbiology

pathogen testing

storage temp.

15-25°C

suitability

Listeria spp.

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This Item
1.103981.401971.02449
GranuCult® FRASER broth (base) pkg of 500 g, according to ISO 11290, APHA, selective for Listeria spp.

1.10398

GranuCult® FRASER broth (base)

suitability

Listeria spp.

suitability

selective for Listeria spp.

suitability

Listeria spp. (spp.)

suitability

Listeria spp. (spp.)

form

medium granules (dehydrated (DCM))

form

medium granules (dehydrated (DCM))

form

-

form

medium granules (dehydrated (DCM))

agency

APHA, according to ISO 11290

agency

APHA, according to ISO 11290

agency

APHA, ISO 11290

agency

APHA, ISO 11290

application(s)

food and beverages
microbiology, pathogen testing

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages, microbiology
pathogen testing

application(s)

food and beverages
microbiology
pathogen testing

manufacturer/tradename

GranuCult®

manufacturer/tradename

GranuCult®

manufacturer/tradename

EcoCult®

manufacturer/tradename

ReadyBag®

General description

GranuCult® Half FRASER broth (base) with antibiotics has high nutrient content and a large buffering capacity that creates optimum growth conditions for Listeria spp. Lithium chloride, nalidixic acid, and acriflavine hydrochloride inhibit the growth of accompanying bacteria. The detection of the β-D-glucosidase activity is possible by adding aesculin and ammonium iron (III) citrate, forming a black complex of aesculin with iron (III) ions. A black coloration can develop during incubation, but this reaction is not exclusive to Listeria spp. Hence, according to EN ISO 11290-1, every primary and secondary enrichment in Fraser broth must be sub-cultured on selective plating media. From TM="GranuCult"] Half FRASER (Demi FRASER) Broth (Base) with Antibiotics primary selective enrichment Half Fraser broth can be prepared by addition of one vial Ammonium iron (III) citrate supplement (1.00092) per 500 mL Half Fraser Broth (base). For the secondary enrichment step, Fraser broth can be prepared by adding one additional vial of reconstituted Fraser Listeria selective supplements (1.00093) per 500 mL Half Fraser Broth.

Application

GranuCult® Half FRASER broth (base) with antibiotics is suitable for the primary selective enrichment of Listeria spp. from food and animal feed as well as from environmental samples and other materials.

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Afterwards confirmation on ALOA®-Medium resp. Tryptic Soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Growth on ALOA medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): >10 colonies
Growth on ALOA medium(Listeria monocytogenes ATCC 35152 (WDCM 00109)): >10 colonies
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021)): blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109)): blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <100 colonies
Incubation:
Half Fraser Broth: 24 ± 2 hours at 30 ± 1°C
Confirmation on ALOA-Medium: 44 ± 4 hours at 37 ± 1°C
Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1°C

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

ALOA is a registered trademark of Biolife Italiana S.r.l.
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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Articles

Microbial culture media is available in both powdered and granulated forms. This article compares the characteristics of each culture media format with regards to safety, handling and convenience.

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

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