1.00191
NutriSelect® prime Bismuth Sulfite (BS) Agar
acc. to Wilson and Blair
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BSA, WILSON BLAIR Agar, Wismuth sulfite agar
Recommended Products
agency
ISO 6579-1
USP 31
Quality Level
description
FDA-BAM
form
powder
technique(s)
microbiological culture: suitable
pH
7.7±0.2 (25 °C)
application(s)
food and beverages
pharmaceutical
storage temp.
2-8°C
suitability
selective and differential for enterobacteriaceae: Salmonella spp.
Related Categories
General description
NutriSelect® Bismuth Sulfite (BS) Agar introduced by Wilson-Blair (1927) is specifically designed for the isolation and differentiation of Salmonella spp from food, animal feed and other materials precisely when the detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi is of concern.
Colonies of H2S positive Salmonella exhibit blackening due to the formation of iron sulfide. The media selectivity is enhanced by the presence of brilliant green and bismuth sulfite which inhibit the growth of gram-positive bacteria and coliforms in the sample.
Peptone, meat extract and glucose allow good growth. Disodium phosphate acts as a buffer to maintain pH and agar is the solidifying agent.
Colonies of H2S positive Salmonella exhibit blackening due to the formation of iron sulfide. The media selectivity is enhanced by the presence of brilliant green and bismuth sulfite which inhibit the growth of gram-positive bacteria and coliforms in the sample.
Peptone, meat extract and glucose allow good growth. Disodium phosphate acts as a buffer to maintain pH and agar is the solidifying agent.
Application
NutriSelect® Bismuth Sulfite (BS) Agar is used to isolate and differentiate Salmonella spp. from food animal feed and other materials. The medium is used to selectively grow the species when S. typhi and S. paratyphi are expected.
Analysis Note
Salmonella colonies display blackening after 18 hrs of incubation.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificates of Analysis (COA)
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