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70186

Millipore

EMB Agar

NutriSelect® Plus, suitable for microbiology

Synonym(s):

Eosin Methylene Blue Agar

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
dipotassium hydrogen phosphate, 2.0 g/L
eosin Y, 0.4 g/L
lactose, 10.0 g/L
methylene blue, 0.065 g/L
peptone, 10.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.1±0.2 (25 °C)

application(s)

agriculture
cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
microbiology

suitability

slightly selective and differential for Candida spp.
slightly selective and differential for Citrobacter spp.
slightly selective and differential for Enterobacter spp.
slightly selective and differential for Enterococcus spp.
slightly selective and differential for Escherichia coli
slightly selective and differential for Klebsiella spp.
slightly selective and differential for Proteus spp.
slightly selective and differential for Salmonella spp.
slightly selective and differential for Staphylococcus spp.
slightly selective and differential for coliforms
slightly selective and differential for enterobacteriaceae

Application

A versatile solid medium for the differentiation of Eschericha coli and Aerobacter aerogenes, for identifcation of Candida albicans and for the identification of coagulase-positive Staphylococci.

Preparation Note

Suspend 37.5 g in 1 litre of distilled water. Bring to the boil to dissolve completely, sterilize by autoclaving at 121°C for 15 minutes. Cool to 60°C and shake the medium in order to oxidize the methylene blue and to suspend the precipitate.

Other Notes

Recommended by the American Public Health Association (APHA) and the United States Pharmacopeia (USP); Compendium of Methods for the Microbiological Examination of Foods; Enumeration of E. coli from lake water.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5692
BCCH3690
BCCG1812
BCCD6329
BCCB6959

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Y Henis et al.
Microbial ecology, 17(2), 171-180 (1989-03-01)
The population of a strain ofEscherichia coli that was resistant to nalidixic acid and streptomycin declined rapidly in samples of sterile and nonsterile Cayuga Lake water and reached an undetectable level in nonsterile water at 24 and 72 hours when
Shuaiguo Zhao et al.
Lab on a chip, 19(6), 941-947 (2019-02-01)
Microfluidic-based portable devices for stool analysis are important for detecting established biomarkers for gastrointestinal disorders and understanding the relationship between gut microbiota imbalances and various health conditions, ranging from digestive disorders to neurodegenerative diseases. However, the challenge of processing stool
J.E. Holt-Harris et al.
The Journal of Infectious Diseases, 18, 596-596 (1916)
Isolation and identification of coagulase-positive Staphylococci on Levine's eosin-methylene blue agar.
N J MENOLASINO
The Journal of laboratory and clinical medicine, 56, 908-910 (1960-12-01)
M. Levine
The Journal of Infectious Diseases, 23, 43-43 (1918)

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