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1.03858

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GranuCult® plus Eosin Methylene Blue Agar

acc. HOLT-HARRIS and TEAGUE; For the isolation and differentiation of E. coli and other gram-negative bacteria

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Synonym(s):
EMB agar, Holt-Harris & Teague (HHT) EMB agar, Eosin Methylene Blue Lactose Sucrose agar

Quality Level

form

powder

storage condition

dry media

technique(s)

microbiological culture: suitable

pH

7.1±0.2 (25 °C)

application(s)

food and beverages
pharmaceutical

storage temp.

15-25°C

suitability

selective for coliforms: Citrobacter spp.
selective for enterobacteriaceae (Genus: Proteus Vulgaris)

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This Item
1.038571.052671.00215
suitability

selective for coliforms: Citrobacter spp., selective for enterobacteriaceae (Genus: Proteus Vulgaris)

suitability

selective and differential for enterobacteriaceae

suitability

Bacillus cereus

suitability

selective and differential for Salmonella spp., selective and differential for Shigella spp.

form

powder

form

powder

form

medium granules (dehydrated (DCM))

form

granular (dehydrated culture medium)

pH

7.1±0.2 (25 °C)

pH

7.1±0.2 (25 °C)

pH

7.0-7.4 (25 °C, 43 g/L in H2O, after autoclaving)

pH

7.5-7.9 (25 °C, 75 g/L in H2O)

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

application(s)

food and beverages
pharmaceutical

application(s)

food and beverages
pharmaceutical

application(s)

food and beverages, microbiology

application(s)

clinical
food and beverages
industrial qc
pharmaceutical, microbiology

General description

GranuCult® plus EMB Agar acc. HOLT-HARRIS and TEAGUE is a low selective and differential medium containing lactose and sucrose and two indicator dyes, Eosin and Methylene Blue. The use of these two indicators allows differentiation between colonies of lactose and/or sucrose fermenting and non-fermenting organisms. Sucrose is included since certain members of the coliform group of microorganism ferment sucrose more readily than lactose. This formulation does not discriminate between carbohydrate utilization (lactose or sucrose).
The peptones provide nitrogen, minerals and amino acids, lactose and sucrose provide the carbon sources. Di-potassium phosphate acts as a buffer whilst Eosin yellowish (Eosin Y) and methylene blue serve as differential indicators and inhibitors. Agar is the solidifying agent.
Coliforms produce blue-black colonies due to the incorporation of an eosin-methylene blue dye complex when the pH drops down. Colonies of Escherichia coli may show a characteristic green metallic luster due to the rapid fermentation of lactose. Lactose- and sucrose-non fermenting bacteria (like most Salmonella and Shigella) will form colourless or transparent amber-coloured colonies, but some strains of Salmonella and Shigella may not grow on this culture medium. Some Gram-positive bacteria and yeasts may grow on this culture medium and usually form pinpoint colonies.

Application

GranuCult® plus Eosin Methylene Blue Agar is a low selective, differential agar used to identify coliforms especially E. coli. It differentiates lactose fermenters from non-lactose fermenters.It is often the media used when testing the potability of water via the MPN method

Features and Benefits

  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to- batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allowing many supplements to be included, with no need to add these separately

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


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