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GranuCult® prime Levine Eosin-Methylene Blue (L-EMB) Agar

ISO 21150, acc. FDA-BAM

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Synonym(s):
EMB agar, LEVINE, Eosin Methylene-blue Lactose Agar acc. to LEVINE

agency

ISO 21150

Quality Level

form

powder

technique(s)

microbiological culture: suitable

pH

7.1±0.2 (25 °C)

application(s)

food and beverages
pharmaceutical

storage temp.

15-25°C

suitability

selective and differential for enterobacteriaceae

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1.038581.002131.03871
agency

ISO 21150

agency

-

agency

ISO 16654

agency

ISO 10273

suitability

selective and differential for enterobacteriaceae

suitability

selective for coliforms: Citrobacter spp., selective for enterobacteriaceae (Genus: Proteus Vulgaris)

suitability

selective and differential for Escherichia 0157:H7

suitability

selective for (Microorganism: Yersinia)

form

powder

form

powder

form

powder

form

powder

pH

7.1±0.2 (25 °C)

pH

7.1±0.2 (25 °C)

pH

7.1±0.2 (25 °C)

pH

7.4±0.2 (25 °C)

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

General description

GranuCult® prime Eosin Methylene Blue (L-EMB) Agar is a nutrient rich medium which is designed for the isolation, confirmation, and identification of Escherichia coli, and other Enterobacteriaceae from the food chain, cosmetic and other materials.
The synergistic effect of lactose and two indicator dyes, Eosin Y and Methylene Blue differentiates colonies of lactose fermenting and non-fermenting microbes. Growing colonies of E. coli produce a green metallic sheen due to high acid production from lactose. Other coliforms do not produce enough acid to attain the metallic sheen. The growing coliforms produce blue-black colonies due to the formation of eosin-methylene blue dye complex when the pH drops.
Peptones in the medium provide nitrogen, amino acids, and minerals to support growth. Di-potassium phosphate acts as a buffer and agar is the solidifying agent.

Application

GranuCult® prime Eosin Methylene Blue (L-EMB) Agar is used for the isolation, confirmation, and identification of Escherichia coli , and other Enterobacteriaceae from the food chain, cosmetic and other materials.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


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