MilliporeSigma
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Millipore

LEVINE-EMB-agar

for the isolation and differentiation of E.coli and Enterobacter

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Quality Level

form

powder (dehydrated (DCM))

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

7.1 (37 °C, 36 g/L in H2O, after autoclaving)

solubility

36 g/L

bulk density

630 kg/m3

application(s)

clinical
microbiology

storage temp.

15-25°C

suitability

Enterobacter spp.
Escherichia coli

General description

for the isolation and differentiation of E.coli and Enterobacter

Analysis Note

Appearance (clearness)clear to almost clearAppearance (colour)red-brownpH-value (25 °C)6.9 - 7.3This medium always contains fine precipitate to a varying extent.Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))≥ 50 %Recovery on test medium (Escherichia coli ATCC 11775 (WDCM 00090))≥ 50 %Recovery on test medium (Escherichia coli 194)≥ 50 %Recovery on test medium (Enterobacter cloacae ATCC 13047 (WDCM 00083))≥ 50 %Growth (Shigella sonnei ATCC 11060)good to very goodGrowth (Salmonella typhimurium ATCC 14028 (WDCM 00031))good to very goodGrowth (Proteus mirabilis ATCC 14273)good to very goodGrowth (Staphylococcus aureus ATCC 25923 (WDCM 00034))none to poorMetallic lustre (Escherichia coli ATCC 25922 (WDCM 00013))+Metallic lustre (Escherichia coli ATCC 11775 (WDCM 00090))+Metallic lustre (Escherichia coli 194)+Metallic lustre (Enterobacter cloacae ATCC 13047 (WDCM 00083))-Metallic lustre (Shigella sonnei ATCC 11060)-Metallic lustre (Salmonella typhimurium ATCC 14028 (WDCM 00031))-Metallic lustre (Proteus mirabilis ATCC 14273)-Incubation: 24 hrs. 35 °C.
E.coli and E.cloacae tested with spiral plate methode, other strains with three-loop-streak.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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