137151

2-Mercaptoethanol

EMPROVE^® EVOLVE

• Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein/REAXYS Number: 773648
• MDL number: MFCD00004890
• UNSPSC Code: 12161504

Properties

• vapor density: 2.69 (vs air)
• Quality Level: 400
• vapor pressure: 1 mmHg ( 20 °C)
• form: liquid
• expl. lim.: 18 %
• concentration: 14.3 M (pure liquid); 99.0-101.0 % (w/w)
• impurities: ≤1.0% related substances (GC) 2,2-Dithiodiethanol
• refractive index: n20/D 1.500 (lit.)
• bp: 157 °C (lit.)
• density: 1.114 g/mL at 25 °C (lit.)
• suitability: corresponds for identity (IR)
• application(s): pharma/biopharma processes
• storage temp.: 2-8°C
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

General description

Our SAFC^® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

As part of our EMPROVE^® Program, our raw materials are offered with EMPROVE^® Dossiers which provide comprehensive, up-to-date documentation to help you navigate regulatory challenges, manage risks, and improve your manufacturing processes.

Our comprehensive portfolio of upstream process chemicals not only provides biopharmaceutical manufacturers with high-quality raw materials for production of classical and novel therapies, but also helps them get to market faster and simplify regulatory challenges. Trust us to deliver supply chain transparency and reliable sourcing around the globe, streamlining your product qualification with best-in-class regulatory support and service.

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Analysis Note

purity (GC) ≥99.0%

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS05,GHS06,GHS08,GHS09
• signalword: Danger
• hcodes: H301 + H331 - H310 - H315 - H317 - H318 - H361fd - H373 - H410
• pcodes: P201 - P260 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P305 + P351 + P338 + P310
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• Storage Class: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 3
• flash_point_c: 74 °C - closed cup