218783

Caspase-1, Human, Recombinant, E. coli

Caspase-1, Human, Recombinant, is expressed in E. coli and is fused at the N-terminus to a His•Tag sequence.

• Synonym(s): IL-1β Converting Enzyme, ICE
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• assay: ≥90% (SDS-PAGE)
• form: liquid
• specific activity: ≥25,000 units/mg protein; ≥50,000 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Research area: APOPTOSIS

Caspases are enzymes that belong to the cysteine protease family. [1] In mammals, researchers have discovered 13 caspases. [2] They enhance chemical reactions by targeting specific substances, guided by aspartic acid. These caspases can be further classified into two groups: apoptotic caspases (caspase-2, 3, 6, 7, 8, 9, and 10) and inflammatory caspases (caspase-1, 4, 5, 11, and 12). [1] Apart from inflammatory organs such as the spleen, lymph nodes, and thymus, caspase-1 is also expressed in adipose tissue, liver, and intestine. [3] Recombinant, human caspase-1 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.

Application

Caspase-1 enzyme has been used in mediating the proteolysis of pro-IL-1β for regulating the availability and activation of IL-1β. [4]

Biochem/physiol Actions

Caspases belong to the cysteine proteases family of enzymes that are responsible for regulating apoptosis and pyroptosis, a programmed necrosis. [2] The activation of caspase-1 in inflammasomes initiates an inflammatory response for fighting infections or cellular damage. [2][1] Furthermore, caspase-1 activated interleukin-1 beta (IL-1β) and interleukin-18 (IL-18) are related to various metabolic disorders such as obesity, atherosclerosis, impairment of insulin action and/or secretion, cancer, and nonalcoholic fatty liver disease. [1] Caspase-1 deficiency could play a role in promoting the growth of tumor cells in colon, ovarian, and prostate cancers. [1]

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-pNA (Cat. No. 400025) per min at 30°C, pH 7.4.

Physical form

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Thornberry, N.A. 1994. Methods Enzymol. 244, 615.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2