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Caspase-1, Human, Recombinant, E. coli

Caspase-1, Human, Recombinant, is expressed in E. coli and is fused at the N-terminus to a His•Tag sequence.

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Synonym(s):
IL-1β Converting Enzyme, ICE

recombinant

expressed in E. coli

Quality Level

assay

≥90% (SDS-PAGE)

form

liquid

specific activity

≥25,000 units/mg protein
≥50,000 units/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

dry ice

storage temp.

−70°C

General description

Research area: APOPTOSIS

Caspases are enzymes that belong to the cysteine protease family. In mammals, researchers have discovered 13 caspases. They enhance chemical reactions by targeting specific substances, guided by aspartic acid. These caspases can be further classified into two groups: apoptotic caspases (caspase-2, 3, 6, 7, 8, 9, and 10) and inflammatory caspases (caspase-1, 4, 5, 11, and 12). Apart from inflammatory organs such as the spleen, lymph nodes, and thymus, caspase-1 is also expressed in adipose tissue, liver, and intestine. Recombinant, human caspase-1 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.

Application

Caspase-1 enzyme has been used in mediating the proteolysis of pro-IL-1β for regulating the availability and activation of IL-1β.

Biochem/physiol Actions

Caspases belong to the cysteine proteases family of enzymes that are responsible for regulating apoptosis and pyroptosis, a programmed necrosis. The activation of caspase-1 in inflammasomes initiates an inflammatory response for fighting infections or cellular damage. Furthermore, caspase-1 activated interleukin-1 beta (IL-1β) and interleukin-18 (IL-18) are related to various metabolic disorders such as obesity, atherosclerosis, impairment of insulin action and/or secretion, cancer, and nonalcoholic fatty liver disease. Caspase-1 deficiency could play a role in promoting the growth of tumor cells in colon, ovarian, and prostate cancers.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-pNA (Cat. No. 400025) per min at 30°C, pH 7.4.

Physical form

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Thornberry, N.A. 1994. Methods Enzymol. 244, 615.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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