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Cathepsin D Substrate I

Useful as a substrate for the determination of cathepsin D activity.

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Synonym(s):
Cathepsin D Substrate I, Bz-RGFFP-4-MeOβNA, HCl
Empirical Formula (Hill Notation):
C49H55N9O7 · xHCl
Molecular Weight:
882.02 (free base basis)

Quality Level

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

white

solubility

DMSO: 5 mg/mL

fluorescence

λex ~345 nm
λem ~425 nm

shipped in

ambient

storage temp.

−20°C

General description

A useful substrate for colorimetric or fluorometric assay of cathepsin D activity. Cathepsin D hydrolyzes the Phe-Phe bond. Dipeptidyl aminopeptidase II is required to free the 4-methoxy-β-naphthylamine (4MβNA). Excitation max: ~345 nm; emission max: ~425 nm.
Useful as a substrate for the determination of cathepsin D activity. Suitable for either colorimetric or fluorometric assay. Cathepsin D hydrolyzes the Phe-Phe bond. Requires dipeptidyl aminopeptidase II to free 4-methoxy-β-naphthylamine (4MeOβNA).

Biochem/physiol Actions

Cell permeable: no
Primary Target
Substrate for the determination of cathepsin D activity
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Bz-Arg-Gly-Phe-Phe-Pro-4MeOβNA, HCl

Other Notes

Takahashi, T., and Tang, J. 1981. Methods Enzymol.80, 565.
Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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