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Cathepsin D/E Substrate, Fluorogenic

An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E, but not cathepsins B, H, or L .

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Synonym(s):
Cathepsin D/E Substrate, Fluorogenic, MOCAc-GKPILF~FRLK(Dnp)- D-R-NH₂, MOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
Empirical Formula (Hill Notation):
C85H122N22O19
Molecular Weight:
1756.01

Quality Level

assay

≥97% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

yellow

solubility

DMSO: 5 mg/mL

fluorescence

λex ~328 nm
λem ~393 nm

shipped in

ambient

storage temp.

2-8°C

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219399219555219392
Sigma-Aldrich

219399

Cathepsin D Substrate I

form

lyophilized solid

form

lyophilized solid

form

solid

form

lyophilized solid

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze, protect from light, desiccated (hygroscopic)

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, desiccated, protect from light

General description

An internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.
An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Substrate for cathepsins D and E
Product does not compete with ATP.
Reversible: no
kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂

MOCAc = (7-Methoxycoumarin-4-yl)acetyl

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Yasuda, Y., et al. 1999. J. Biochem.126, 260.
Yasuda, Y., et al. 1999. J. Biochem.125, 1137.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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