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539735

Sigma-Aldrich

PTP1B, Human, Recombinant, E. coli

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Synonym(s):
Protein Tyrosine Phosphatase 1B

assay

≥90% (SDS-PAGE)

Quality Level

form

liquid

specific activity

≥20 U/mg

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

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539568553325539514
Sigma-Aldrich

539735

PTP1B, Human, Recombinant, E. coli

Sigma-Aldrich

539568

PP2B, Human, Recombinant, E. coli

form

liquid

form

liquid

form

liquid

form

liquid

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

specific activity

≥20 U/mg

specific activity

≥300,000 U/mg, ≥50,000 units/mL

specific activity

-

specific activity

≥400,000 units/mL

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Recombinant, human PTP1B expressed in E. coli. PTP1B, an ubiquitous nontransmembrane protein tyrosine phosphatase, was originally identified in human placenta. This highly active enzyme is useful for the study of tyrosine phosphatase kinetics, substrate specificity, and for screening inhibitors.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphopeptide substrate (Cat. No. 539737) per min at 30°C, pH 7.2 using 150 µM of substrate.

Physical form

In 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.05% NP-40, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Puius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA94, 13420.
Liu, F., et al. 1996. J. Biol. Chem.271, 31290.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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