P6244

Protein Tyrosine Phosphatase 1B human

recombinant, expressed in E. coli

• Synonym(s): PTP-1B
• MDL number: MFCD01635664
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: solution
• specific activity: ≥20 units/mg protein
• mol wt: 37.4 kDa
• concentration: ≥0.5 mg/mL
• UniProt accession no.: P18031
• relevant disease(s): diabetes; obesity; cancer
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Protein tyrosine phosphatase 1B human (PTP1B) is encoded by the gene PTPN1. In human chromosome, the gene PTPN1 is localized on 20q13.13.[1] The protein has 37 kDa catalytic domain and the C-terminal hydrophobic residues targets the protein to the cytoplasmic side of the endoplasmic reticulum membrane.[2]

Application

Protein Tyrosine Phosphatase 1B (PTP1B) human has been used in treating cell lysate prior to immunoprecipitation and protein pull-down studies.[3] It has also been used in PTP1B activity assay and also for the evaluation of inhibitors like betulinic acid (BA) and sodium orthovanadate (Na3VO4),[4] and 6-chloro-3-formyl-7-methylchromone (CFM) on PTP1B.[5]

Protein tyrosine phosphatase 1B (PTP1B) is a pharmaceutical target used to study the treatment of various diseases such as obesity, cancer and type II diabetes mellitus[6].

The inhibitory effect of 8-(4-chlorophenylthio)-cAMP and 8-(4-chlorophenylthio)-cGMP on protein tyrosine phosphatase-1B has been assayed.[7]

Biochem/physiol Actions

PTP-1B is a recombinant, non-transmembrane protein expressed in E. coli containing amino acid residues 1-322 of human PTP-1B. It has a molecular mass of 37.4 kDa.[8][9] It is an abundant intracellular enzyme that is thought to act as a negative regulator of certain signaling pathways.[8] It dephosphorylates tyrosine-phosphorylated proteins and peptides, and is usually localized in the cytostolic domain of the ER.

PTP1B dephosphorylates c-Src in several human breast cancer cell lines has a regulatory role for PTP1B in the control of c-Src kinase activity[6]. PTP1B is regulates the cardiovascular effects of leptin[10]. PTP1B controls the JAK/STAT signaling pathway by dephosphorylation of JAK2[10].

Polymorphism in the gene PTPN1 is associated with type 2 diabetes and obesity.[1]

Unit Definition

One unit will hydrolyze 1.0 μmole of a phosphopeptide, EGFR fragment 988-998, per minute at pH 7.2 at 30 °C

Physical form

Supplied as a solution in 50 mM HEPES (pH 7.2), 1 mM EDTA, 5 mM DTT, and 0.05% NP-40

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable