648465

Triton^™ X-100 Detergent, Hydrogenated

Nonionic detergent used for the solubilization of membrane-bound proteins.

• Synonym(s): Octylphenoxypolyethoxyethanol, Hydrogenated, TRX-100
• CAS Number: 92046-34-9
• UNSPSC Code: 12161902
• NACRES: NA.25

Properties

• Quality Level: 100
• form: viscous liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: colorless
• CMC: 250 μM
• solubility: water: 10%
• shipped in: ambient
• storage temp.: 15-25°C
• InChI: 1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3
• InChI key: QQJNBKDKLMCALZ-UHFFFAOYSA-N

Description

General description

Nonionic detergent used for the solubilization of membrane-bound proteins. Lacks the UV absorbance and fluorescence of TRITON^™ X-100 (Cat. No. 648462), which causes interference in spectophotometric monitoring and assays. Has a critical micelle concentration (CMC) of 250 µM.

Application

• The utilization of Triton X-100 for enhanced two-dimensional liquid-phase proteomics.: This study explores the role of Triton X-100 in enhancing protein separation techniques in biochemistry, particularly emphasizing its application in two-dimensional liquid-phase proteomics to improve resolution and reproducibility (Kim et al., 2011).


• Effects of environment on the photophysical characteristics of mesotetrakis methylpyridiniumyl porphyrin (TMPyP).: Research focuses on the influence of different solvent environments, including those containing Triton X-100, on the photophysical properties of porphyrins, highlighting its utility in enhancing solubility and stability in biochemical assays (Gonccedil;alves et al., 2011).


• Processing of tissue culture cells.: The work describes methods for processing tissue culture cells where Triton X-100 is used to permeabilize cells for various staining procedures, crucial for molecular biology and cellular biochemistry studies (Bratthauer, 2010).

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Reconstitution

Following dilution in H₂O, store at room temperature. Diluted stock solutions are stable for up to 3 months at room temperature.

Other Notes

Fernandez, J., et al. 1992. Anal. Biochem.201, 255.
Tiller, G.E., et al. 1984. Anal. Biochem.141, 262.
Ashani, Y. and Cantravas, G.N. 1980. Anal. Biochem.109, 55.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H315,H319,H335,H411
• pcodes: P261 - P264 - P271 - P273 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: 235.4 °F - closed cup
• flash_point_c: 113 °C - closed cup