AB1779SP
Anti-Brain Derived Neurotrophic Factor Antibody
Chemicon®, from rabbit
Synonym(s):
BDNF
About This Item
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BDNF(627)
Related Categories
Specificity
Immunogen
application
Immunoblotting: 5-10μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C. BDNF migrates as 18kDa, with a homodimer at ~27-30kDa. 1:1,000
ELISA: 5-10μg/mL
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Optimal working dilutions must be determined by end user.
Neuroscience
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Linkage
Physical form
Storage and Stability
Legal Information
Disclaimer
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recommended
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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