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AB5300

Sigma-Aldrich

Anti-Amyloid Precursor Protein Antibody, universal

serum, Chemicon®

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Synonym(s):
APP
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, monkey, mouse

should not react with

bovine

manufacturer/tradename

Chemicon®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... APP(351)

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This Item
AB5352AB1593AB5302
antibody form

serum

antibody form

serum

antibody form

serum

antibody form

serum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, monkey, mouse

species reactivity

human, monkey

species reactivity

human

species reactivity

monkey, mouse, human, bovine

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

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Specificity

Amyloid Precursor Protein (APP), Universal. Specific to amino acids 99-126.

Immunogen

APP (aa 99-126) conjugated to KLH.
Epitope: universal

Application

Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-Amyloid Precursor Protein Antibody, universal is validated for use in IP, WB for the detection of Amyloid Precursor Protein.
Western blot: 1:250 - 1:1,000: need to reduce and alkylate disulfide bonds of APP to expose the epitope on APP transferred to nitrocellulose or PVDF prior to immunoblotting.

To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.

Immunoprecipitation: Need to reduce and alkylate disulfide bonds to expose the epitope

Optimal working dilutions must be determined by end user.

Physical form

Serum. Liquid in PBS with 0.01% azide.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Zhi-Xing Yao et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 16(12), 1677-1679 (2002-09-19)
Amyloid beta-peptide (Abeta), Abeta precursor protein (APP), apolipoprotein E (apoE), and elevated cholesterol levels have been linked to Alzheimer's disease (AD) pathology. High cholesterol levels increase APP and apoE expression in human NT2 neuron progenitor cells. A cholesterol-rich environment also
Fuyan Chen et al.
Experimental and therapeutic medicine, 20(5), 88-88 (2020-09-26)
Alzheimer's disease (AD), the leading cause of age-related dementia, is characterized by abnormal β-amyloid accumulation. During learning, memory formation and consolidation, increased levels of histone H3 and H4 acetylation are observed. The present study reported significantly decreased level of H4K16ac
A-Ryeong Gwon et al.
Aging cell, 11(4), 559-568 (2012-03-13)
The cause of elevated level of amyloid β-peptide (Aβ42) in common late-onset sporadic [Alzheimer's disease (AD)] has not been established. Here, we show that the membrane lipid peroxidation product 4-hydroxynonenal (HNE) is associated with amyloid and neurodegenerative pathologies in AD

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