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Key Documents

AB9598

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Antibody

CHEMICON®, rabbit polyclonal

Synonym(s):

GFAPdelta

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-Glial fibrillary acidic protein δ Antibody, serum, Chemicon®

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

should not react with

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)

General description

Human Glial Fibrillary Acidic Protein delta (GFAP-delta) is a GFAP protein isoform that is encoded by an alternative splice variant of the GFAP-gene. As a result, GFAP-delta protein differs from the predominant splice form, GFAP-alpha, by its C-terminal protein sequence. GFAP-delta protein is not expressed by all GFAP expressing astrocytes but specifically by a subpopulation located in the subpial zone of the cerebral cortex, the subgranular zone of the hippocampus and, most intensely, by a ribbon of astrocytes following the ependymal layer of the cerebral ventricles. Therefore, at least in the sub ventricular zone (SVZ), GFAP-delta specifically marks the population of astrocytes that contain the neural stem cells in the adult human brain. Interestingly, the SVZ astrocytes actively splice GFAP-delta transcripts, in contrast to astrocytes adjacent to this layer. Data shows that GFAP-delta protein, unlike GFAP-alpha, is not upregulated in astrogliosis. Data indicates a different functional role for GFAP-delta in astrocyte physiology. Transfection studies showed that GFAP-delta protein expression has a negative effect on GFAP filament formation, and therefore could be important for modulating intermediate filament cytoskeletal properties, possibly facilitating astrocyte motility. Further studies on GFAP-delta and the cells that express it are important for gaining insights into its function during differentiation, migration and during health and disease (Roelofs, RF, et al., Glia (2005) 52:289-300.).

Specificity

Glial Fibrillary Acidic Protein-delta (GFAP-delta). The antibody does not recognize GFAP-alpha protein.

Immunogen

Synthetic peptide from human GFAP-delta.

Application

Anti-Glial fibrillary acidic protein δ Antibody is an antibody against Glial fibrillary acidic protein δ for use in WB, IH(P).
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Western blot: 1:500 with overnight incubation. The antibody recognizes the ~60 kDa GFAP-delta protein.

Immunohistochemistry: 1:500 incubated for 36-48 hours at 2-8°C on paraffin embedded tissue sections. It is suggested that the tissue be treated with microwave antigen retrieval prior to staining.

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: 04-1031; 04-1062

Physical form

Rabbit serum. Liquid.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Mark J Swanson et al.
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There are reports that astrocyte perivascular endfeet are damaged in some cases of multiple sclerosis (MS). This study was designed to determine the origin and outcome of astrocyte damage in acute, resolving, and inactive plaques. Ten acute plaques from 10
Olig2-induced neural stem cell differentiation involves downregulation of Wnt signaling and induction of Dickkopf-1 expression.
Ahn, SM; Byun, K; Kim, D; Lee, K; Yoo, JS; Kim, SU; Jho, EH; Simpson, RJ; Lee, B
Testing null
Sara Cipriani et al.
Cerebral cortex (New York, N.Y. : 1991), 28(7), 2458-2478 (2018-05-04)
Neuropathological conditions might affect adult granulogenesis in the adult human dentate gyrus. However, radial glial cells (RGCs) have not been well characterized during human development and aging. We have previously described progenitor and neuronal layer establishment in the hippocampal pyramidal
Brian W Leonard et al.
The Journal of comparative neurology, 515(3), 269-294 (2009-05-09)
In mice and in young adult humans, the subventricular zone (SVZ) contains multipotent, dividing astrocytes, some of which, when cultured, produce neurospheres that differentiate into neurons and glia. It is unknown whether the SVZ of very old humans has this

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