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ABF23

Sigma-Aldrich

Anti-NLRP3 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
NACHT, LRR and PYD domains-containing protein 3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, CLR1.1, Cold autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, NLRP3
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NLRP3(114548)

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This Item
SAB5700813SAB1410191HPA012878
Anti-NLRP3 Antibody from rabbit, purified by affinity chromatography

ABF23

Anti-NLRP3 Antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

Gene Information

human ... NLRP3(114548)

Gene Information

human ... NLRP3(114548)

Gene Information

human ... NLRP3(114548)

Gene Information

human ... NLRP3(114548)

species reactivity

human

species reactivity

human, mouse, rat

species reactivity

human

species reactivity

human

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General description

NLRPs (Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing Proteins) are members of NLR (Nod-like receptors) protein family. NRLP genes are known to play important roles in both the mammalian reproductive system and the innate immune system. NLRP3 may function as an inducer of apoptosis, activator of NF-kappa-B signaling and activates caspase-1 in response to triggers such as bacterial or viral infection. NLRP3 defects are linked to familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA). NLRP3 is highly expressed in blood leukocytes, polymorphonuclear cells, osteoblasts and chondrocytes.

Specificity

Other homologies: Porcine (80% sequence homology).
This antibody recognizes the DAPIN domain of NLRP3.

Immunogen

Epitope: DAPIN domain
KLH-conjugated linear peptide corresponding to the DAPIN domain of human NLRP3.

Application

Detect NLRP3 using this rabbit polyclonal antibody, Anti-NLRP3 Antibody validated for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected NLRP3 in human leukemia bone marrow and human tonsil tissue lysate.
Research Category
Inflammation & Immunology
Research Sub Category
Immunological Signaling

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NLRP3 in 10 µg of THP-1 cell lysate.

Target description

~112 kDa and ~75 kDa observed. Five isoforms are known to exist at 118 kDa, 112 kDa, 112 kDa, 106 kDa, and 84 kDa This protein has been observed at ~75 kDa in some cell lysates (Kummer, J. A., et al. (2007). J Histochem Cytochem. 55(5):443-452.). Uncharacterized bands may be observed in some cell lysates.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
THP-1 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Ching-Wei Wang et al.
Cancers, 14(7) (2022-04-13)
Ovarian cancer is a common malignant gynecological disease. Molecular target therapy, i.e., antiangiogenesis with bevacizumab, was found to be effective in some patients of epithelial ovarian cancer (EOC). Although careful patient selection is essential, there are currently no biomarkers available
Mélanie Bodnar-Wachtel et al.
Life science alliance, 6(4) (2023-02-07)
NLRP3 is a pattern recognition receptor with a well-documented role in inducing inflammasome assembly in response to cellular stress. Deregulation of its activity leads to many inflammatory disorders including gouty arthritis, Alzheimer disease, and cancer. Whereas its role in the

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