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ABT1485-AF647

Sigma-Aldrich

Anti-Actin Antibody, Alexa Fluor 647 Conjugate

from rabbit, ALEXA FLUOR 647

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Synonym(s):
Actin, alpha skeletal muscle, Alpha-actin-1
eCl@ss:
32160702

biological source

rabbit

Quality Level

conjugate

ALEXA FLUOR 647

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... ACTA1(58)

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This Item
ABT1485-AF488CBL171-IMABT824
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

mouse

conjugate

ALEXA FLUOR 647

conjugate

ALEXA FLUOR 488

conjugate

-

conjugate

-

UniProt accession no.

P68133

UniProt accession no.

P68133

UniProt accession no.

P62736

UniProt accession no.

P63261

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunocytochemistry: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

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General description

Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show more than 90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.

Specificity

Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.
This Alexa Fluor 647 conjugate was prepared from purified rabbit polyclonal antibody (Cat. No. ABT1485) raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).

Application

Flow Cytometry Analysis: 2 µL from a representative lot detected actin in one million HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in NIH/3T3 cells.

Immunofluorescence Analysis: A 1:50-150 dilution from a representative lot detected actin in human heart, prostate, skeletal muscle, and small intestine tissue sections.

The unconjugated antibody (Cat. No. ABT1485) and Alexa Fluor 488-conjugated antibody (Cat. No. ABT1485-AF488) are also available for flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry and Western blotting applications.
Research Category
Cell Structure
This rabbit polyclonal Anti-Actin, Alexa Fluor 647 Conjugate, Cat. No. ABT1485-AF647 s validated for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence applications for the detection of Actin.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in A431 cells.

Target description

~42 kDa observed.

Physical form

Affinity purified.
Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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