MilliporeSigma
All Photos(2)

Documents

CBL171

Sigma-Aldrich

Anti-Actin Antibody, smooth muscle, clone ASM-1

clone ASM-1, Chemicon®, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Alpha-actin-2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ASM-1, monoclonal

species reactivity

chicken, mouse, horse, rat, human, bovine

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ACTA2(59)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A5228A2547SAB4200679
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

ASM-1, monoclonal

clone

1A4, monoclonal

clone

1A4, monoclonal

clone

1A4, monoclonal

species reactivity

chicken, mouse, horse, rat, human, bovine

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

species reactivity

frog, mouse, hamster, chicken, viper, bovine, crayfish, goat, rat, cat, sheep, guinea pig, lizard, human, rabbit, dog, snail

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable using smooth muscle cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells, indirect ELISA: suitable, indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections, microarray: suitable, western blot: suitable using smooth muscle cells

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells, immunohistochemistry (frozen sections): suitable using smooth muscle cells, indirect immunofluorescence: 1:400 using blood vessels in sections of human appendix, western blot: suitable using smooth muscle cells

technique(s)

direct ELISA: suitable, immunoblotting: 2-4 μg/mL using extracts of mouse heart tissues, immunohistochemistry: 5-10 μg/mL using human appendix or tonsil tissue sections

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.

Specificity

Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed.
This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).

Immunogen

Epitope: N-terminus
Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.

Application

Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.
Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.

Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.

Target description

43 kDa

Linkage

Replaces: 04-1040

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Constantin C Chipev et al.
BMC dermatology, 2, 13-13 (2002-11-26)
Wounds in the nonglabrous skin of keloid-prone individuals tend to cause large disordered accumulations of collagen which extend beyond the original margins of the wound. In addition to abnormalities in keloid fibroblasts, comparison of dermal fibroblasts derived from nonwounded glabrous
Manuela Mura et al.
Stem cell research, 39, 101510-101510 (2019-08-10)
We generated PSMi001-A and PSMi008-A hiPSC lines from two individuals belonging to a South African (SA) founder population in which the malignant KCNQ1-A341V mutation cosegregates with the Long QT Syndrome (LQTS) phenotype. PSMi001-A was derived from an asymptomatic KCNQ1-A341V mutation
Junfeng Zhang et al.
PloS one, 8(12), e83294-e83294 (2013-12-19)
Endothelial progenitor cells (EPCs) are capable of proliferating and differentiating into mature endothelial cells, and they have been considered as potential candidates for coronary heart disease therapy. However, the transition of EPCs to mesenchymal cells is not fully understood. This
Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature.
Thomas, SM; Kagan, C; Pavlovic, BJ; Burnett, J; Patterson, K; Pritchard, JK; Gilad, Y
PLoS Genetics null
Localization of angiopoietin-1 and Tie2 immunoreactivity in rodent ependyma and adjacent blood vessels suggests functional relationships.
Horton, BN; Solanki, RB; Rajneesh, KF; Kulesza, P; Ardelt, AA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service