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CBL186

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Anti-DNA Antibody, clone AC-30-10

clone AC-30-10, Chemicon®, from mouse

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Synonym(s):
Deoxyribonucleic acid
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-30-10, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgM

shipped in

wet ice

target post-translational modification

unmodified

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antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

AC-30-10, monoclonal

clone

AE1, monoclonal

clone

IST-10, monoclonal

clone

7D1.13, monoclonal

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

immunohistochemistry: suitable (paraffin)

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable

isotype

IgM

isotype

IgG

isotype

IgG1κ

isotype

IgG1κ

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Specificity

This antibody reacts with native denatured DNA extracted from various organisms: mouse DNA, calf thymus DNA, M13 DNA, SV-40 DNA and several plasmids. It also reacts with synthetic DNA of different base compositions: poly dAT-poly dAT, poly dAT. DNA-containing structures such as chromatin in diverse tissues from several species as well as in diverse cell lines such as XLKE-A6 (Xenopus), RVF-SMC (rat kangaroo) and HeLa (human) are also recognized.

Application

Anti-DNA Antibody, clone AC-30-10 is a Mouse Monoclonal Antibody for the detection of DNA & has been validated in IF, IHC(P).
Research (cell biology, molecular biology, microbiology) since it reacts with any form of native and denatured DNA. Reliable positive control in immunofluoresence assays for detecting DNA antibodies on tissues of cultured cells, notably in the diagnosis of autoantibodies and autoimmune diseases.

Sensitive detection of mycoplasma contamination in cell cultures.
For frozen and paraffin tissue sections.

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Physical form

Format: Purified
The immunoglobulin was purified by gel filtration and presented in PBS, pH 7.4, containing 0.09% sodium azide and 0.5% BSA.

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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