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MAB1572

Sigma-Aldrich

Anti-Parvalbumin Antibody

ascites fluid, clone PARV-19, Chemicon®

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Synonym(s):
Anti-D22S749
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PARV-19, monoclonal

species reactivity

feline, rabbit, goat, frog, fish, human, pig, bovine, rat, mouse, canine

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PVALB(5816)

General description

Parvalbumin is a calcium binding albumin protein. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is localised in fast-contracting muscles, where its levels are highest, and in the brain and some endocrine tissues. Parvalbumin is present in GABAergic interneurons in the nervous system, predominantly expressed by chandelier and basket cells in the cortex.

Specificity

Parvalbumin. The antibody will react with parvalbumin from brain and muscle. By immunoblot it recognizes a protein of 12 kDa. The antibody is directed against an epitope at the first Ca+2-binding site and specifically stains the Ca+2 -bound form of parvalbumin.

Immunogen

Parvalbumin purified from frog muscle

Application

Anti-Parvalbumin Antibody is an antibody against Parvalbumin for use in IC, IH(P) & WB.
Immunohistochemistry:
A previous lot was used by indirect immunoperoxidase at 1:1,000-1:2,000 dilution. The antibody works on formalin fixed, paraffin embedded tissue sections of rat cerebellum from a previous lot. Fixation does not appear to affect MAB1572’s ability to detect parvalbumin.

Immunocytochemistry:
A previous lot of this antibody was used on cultured neurons.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience

Quality

Routinely evaluated by Western Blot on mouse brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected Parvalbumin on 10 μg of mouse brain lysate.

Target description

12 kDa

Physical form

Ascites mouse monoclonal IgG1 liquid
Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Brain tissue, Cultured neurons

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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