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MAB030

Sigma-Aldrich

Anti-DNA Antibody, double stranded, clone BV16-13

culture supernatant, clone BV16-13, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

BV16-13, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
radioimmunoassay: suitable

isotype

IgG2aκ

shipped in

wet ice

target post-translational modification

unmodified

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Anti-MDR1 Antibody, clone JSB-1 culture supernatant, clone JSB-1, Chemicon®

MAB4120

Anti-MDR1 Antibody, clone JSB-1

Sigma-Aldrich

MAB4148

Anti-MRP2 Antibody, clone M2 I-4

clone

BV16-13, monoclonal

clone

JSB-1, monoclonal

clone

M2 III-6, monoclonal

clone

M2 I-4, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

ELISA: suitable, radioimmunoassay: suitable, immunocytochemistry: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Quality Level

100

Quality Level

300

Quality Level

300

Quality Level

300

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Specificity

Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.

BINDING ASSAY:

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.



ANTIGEN BINDING

PROFILE: Relative Binding To:



Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01



Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).



a) Native calf thymus DNA b) Heat-denatured calf thymus DNA

Immunogen

Epitope: double stranded, 28% reactive with single stranded

Application

Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
EIA/RIA

Immunocytochemistry

Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair

Apoptosis - Additional

Physical form

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Sravani K Ramisetty et al.
International journal of biological macromolecules, 103, 845-853 (2017-05-26)
The macromolecules of the bacterial cell occupy 20-40% of the total cytosol volume, and crowded environments have long been known to compact and stabilize DNA. Nevertheless, investigations on DNA-protein binding are generally performed in the absence of crowding, which may
Rajeeva Singh et al.
Molecular pharmaceutics, 17(1), 50-58 (2019-11-20)
DNA-targeting indolinobenzodiazepine dimer (IGN) payloads are used in several clinical-stage antibody-drug conjugates. IGN drugs alkylate DNA through the single imine moiety present in the dimer in contrast to the pyrrolobenzodiazepine dimer drugs, such as talirine and tesirine, which contain two
Petter Langlete et al.
Frontiers in microbiology, 10, 2708-2708 (2019-12-12)
Extracellular vesicles secreted by Gram-negative bacteria have proven to be important in bacterial defense, communication and host-pathogen relationships. They resemble smaller versions of the bacterial mother cell, with similar contents of proteins, LPS, DNA, and RNA. Vesicles can elicit a
C A Cooke et al.
The Journal of cell biology, 120(5), 1083-1091 (1993-03-01)
The location of the cis-acting DNA sequences that direct the assembly of the mammalian kinetochore is not known. A variety of circumstantial evidence, however, has led to the widespread belief that they are present throughout the kinetochore including the kinetochore
Taeko Sasaki et al.
Scientific reports, 7(1), 11257-11257 (2017-09-14)
Mitochondrial DNA (mtDNA) is organized in nucleoprotein complexes called mitochondrial nucleoids (mt-nucleoids), which are critical units of mtDNA replication and transmission. In humans, several hundreds of mt-nucleoids exist in a cell. However, how numerous mt-nucleoids are maintained during the cell

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