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MAB3868

Sigma-Aldrich

Anti-DNA Antibody, single stranded

clone TNT-3, Chemicon®, from mouse

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Synonym(s):
ssDNA
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TNT-3, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgG1, kappa

shipped in

dry ice

target post-translational modification

unmodified

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ZMS1047MAB3222MAB2074Z
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

TNT-3, monoclonal

clone

AE-2, recombinant monoclonal

clone

IIB5, monoclonal

clone

E7P6, monoclonal

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

dot blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

isotype

IgG1, kappa

isotype

IgG1

isotype

IgG1

isotype

IgG1

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Specificity

Reacts with single stranded DNA (ssDNA). The antibody is useful for staining nucleated cells. The antibody can also be used to identify necrotic regions of tumors and damaged tissues. By immunohistochemistry, stains heterochromatic regions of cell nucleus.

Immunogen

Human Raji cell nuclei

Application

Detect DNA using this Anti-DNA Antibody, single stranded validated for use in ELISA, FC, IC, IH(P).
Immunocytochemistry on paraformaldehyde fixed cells

Immunohistochemistry on paraformaldehyde or B5 fixed tissue sections.

Flow cytometry: 5-10 mg/mL using 2% paraformaldehyde fixed cells.

ELISA

Optimal working dilutions must be determined by end user.

STAINING PATTERN:

Stains heterochromatic regions of cell nucleus.
Research Category
Epitope Tags & General Use
Research Sub Category
Organelle & Cell Markers

Physical form

Format: Purified
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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T1503
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25G
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705578-5MG-PW

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1000309185

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New histone supply regulates replication fork speed and PCNA unloading.
Mejlvang, J; Feng, Y; Alabert, C; Neelsen, KJ; Jasencakova, Z; Zhao, X; Lees, M; Sandelin et al.
The Journal of cell biology null
Francesco Nannini et al.
mAbs, 13(1), 1864084-1864084 (2021-01-01)
Phage display technology in combination with next-generation sequencing (NGS) currently is a state-of-the-art method for the enrichment and isolation of monoclonal antibodies from diverse libraries. However, the current NGS methods employed for sequencing phage display libraries are limited by the
LiJun Wang et al.
Cellular reprogramming, 23(2), 99-107 (2021-04-17)
Zygotic epigenetic reprogramming is the major initial event in embryo development to acquire a totipotent potential. However, the patterns of epigenetic modifications in bovine zygote were not well clarified, especially in the first cell cycle of bovine somatic cell nuclear
Fan Guo et al.
Cell stem cell, 15(4), 447-459 (2014-09-16)
The epigenomes of mammalian sperm and oocytes, characterized by gamete-specific 5-methylcytosine (5mC) patterns, are reprogrammed during early embryogenesis to establish full developmental potential. Previous studies have suggested that the paternal genome is actively demethylated in the zygote while the maternal
Christiane B de Araujo et al.
The Journal of eukaryotic microbiology, 66(3), 514-518 (2018-08-05)
Here, we investigated the features of replication in Trypanosoma cruzi epimastigotes based on fork speed progression, which is influenced by distinct features such as DNA polymerase rate, susceptibility to DNA damage and repair, secondary structures, transcription and chromatin state. Although

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