MilliporeSigma
All Photos(12)

Documents

MAB3299

Sigma-Aldrich

Anti-DNA Antibody, single stranded specific, clone F7-26

clone F7-26, Chemicon®, from mouse

Sign Into View Organizational & Contract Pricing

eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

F7-26, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgM

shipped in

dry ice

target post-translational modification

unmodified

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABF2791MAB4072MAB3034
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

F7-26, monoclonal

clone

7E7, monoclonal

clone

131-14871, monoclonal

clone

16-19, monoclonal

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

ELISA: suitable

isotype

IgM

isotype

IgG2b

isotype

IgG1

isotype

IgG2aκ

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Apoptosis is a genetically and biochemically regulated mechanism of programmed cell death that plays an important role in a variety of human diseases including cancer, immune disorders, and neurologic, cardiovascular and infectious diseases. Decreased stability of apoptotic DNA toward thermal denaturation is induced by the proteolysis of DNA-bound proteins during the execution of apoptosis. This conclusion is supported by the binding of anti-ssDNA monoclonal antibodies to non-apoptotic cells treated with proteinase K before heating and by prevention of DNA denaturation and MAb reactivity in apoptotic nuclei reconstituted with histones (Frankfurt et al. 1996, 1997). Thus, staining of cell suspensions and tissue sections with such antibodies following a heat treatment, which induces DNA denaturation in situ only in apoptotic nuclei, is a specific and sensitive method for the detection of apoptotic cells. CHEMICON MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types.

Specificity

Specifically reactive with single-stranded DNA and is ideal for the detection of apoptotic cells in suspension or in tissue sections. The antibody does not recognize DNA in double-stranded conformations. MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types. Antibody reacts specifically with deoxycytidine and requires stretch of ssDNA of at least 25-30 bases in length for the binding. Importantly, in contrast with the TUNEL method, monoclonal antibodies to ssDNA are specific for apoptotic cell death and do not detect necrotic cells.

Immunogen

Epitope: single stranded specific
F7-26 was generated by the immunization of mice with calf thymus single-stranded DNA.

Application

1. Immunoprecipitation

2. Immunohistochemistry: formalin fixed, paraffin embedded tissues & 4% PF frozen tissues (special detection protocol required. FACS analysis (special protocol required),
Anti-DNA Antibody, single stranded specific, clone F7-26 detects level of DNA & has been published & validated for use in FC, IC, IH, IH(P).

Physical form

Format: Purified
Purified Immunoglobulin. Liquid in PBS supplemented with 5% fetal bovine Serum (FBS), containing no preservatives.

Storage and Stability

Maintain frozen at -80°C in undiluted aliquots for up to 12 months after date of purchase.

Analysis Note

Control
Apoptotic cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Fumiko Kobayashi et al.
Infection and immunity, 79(12), 4791-4801 (2011-09-29)
Helicobacter pylori infection is associated with several autoimmune diseases, in which autoantibody-producing B cells must be activated. Among these B cells, CD5-positive B-1a cells from BALB/c mice were confirmed to secrete autoantibodies when cocultured with purified H. pylori urease in
Jia Chen et al.
Brain research. Molecular brain research, 134(2), 215-225 (2005-04-20)
The neuroprotective effects of Delta(9)-tetrahydrocannabinol (THC) were examined using an in vitro model in which the AF5 CNS cell line was exposed to toxic levels of N-methyl-d-aspartate (NMDA), an agonist of the NMDA glutamate receptor. NMDA toxicity was reduced by
Simon J Fung et al.
Brain research, 1179, 42-50 (2007-09-25)
Patients with obstructive sleep apnea (OSA) exhibit hippocampal damage and cognitive deficits. To determine the effect of apnea on the synaptic transmission in the hippocampus, we performed electrophysiological studies in an in vivo guinea pig model of OSA. Specifically, we
Alan L Chang et al.
Free radical biology & medicine, 78, 179-189 (2014-12-03)
Oxidative mitochondrial damage is closely linked to inflammation and cell death, but low levels of reactive oxygen and nitrogen species serve as signals that involve mitochondrial repair and resolution of inflammation. More specifically, cytoprotection relies on the elimination of damaged
Kiran Assi et al.
Immunology, 118(1), 112-121 (2006-04-25)
Stress-activated protein kinases (SAPKs) are activated in human inflammatory bowel disease (IBD). Recently it has been demonstrated that p38MAPK (mitogen-activated protein kinase) inhibition using SB203580 is effective in reducing disease in both dextran sulphate sodium (DSS)-induced and 2,4,6-trinitrobenzenesulphonic acid (TNBS)-induced

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service