Skip to Content
MilliporeSigma
All Photos(1)

Documents

GF175

Sigma-Aldrich

WNT-1, Human Recombinant

Sign Into View Organizational & Contract Pricing

Synonym(s):
WNT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

assay

>98% (SDS-PAGE and HPLC)

Quality Level

technique(s)

cell culture | stem cell: suitable

impurities

<0.1 μg/mg protein endotoxin (<1EU/μg)

UniProt accession no.

shipped in

dry ice

General description

Product Source: E. coli.
Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.

Specificity

Cross Reactivty
Mouse

Application

Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors

Quality

Bioactivity assay: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.

Physical form

Product is filtered through a 0.2 micron filter before lyophilization. Product is presented in .

Storage and Stability

Store at -20°C for up to 6 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service