HTS215M
ChemiSCREEN Membrane Preparation Recombinant Human 5-HT₇ Serotonin Receptor
Human 5-HT7 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Select a Size
$892.00
Estimated to ship onMarch 23, 2025
Select a Size
About This Item
$892.00
Estimated to ship onMarch 23, 2025
Recommended Products
biological source
human
Quality Level
recombinant
expressed in Chem-1 cells
manufacturer/tradename
ChemiScreen
Chemicon®
technique(s)
ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... HTR7(3363)
1 of 4
This Item | HTS111M | HTS108M | HTS132M |
---|---|---|---|
expressed in Chem-1 cells | expressed in Chem-1 cells | expressed in Chem-1 cells | expressed in Chem-1 cells |
ChemiScreen | ChemiScreen, Chemicon® | ChemiScreen, Chemicon® | ChemiScreen, Chemicon® |
ligand binding assay: suitable (GTPγS) | ligand binding assay: suitable (GTPγS), radioligand binding assay (RLBA): suitable | ligand binding assay: suitable (GTPγS), radioligand binding assay (RLBA): suitable | ligand binding assay: suitable (GTPγS), radioligand binding assay (RLBA): suitable |
General description
Application
Biochem/physiol Actions
Quality
10 µg/well | |
---|---|
Signal:Background | 7.5 |
Specific Binding (cpm) | 545 |
SPECIFICATIONS: 1 unit =10 μg
Bmax for [³H]-5-CT binding: 20.1 pmol/mg protein
Kd for [³H]-5-CT binding: ~37.7 nM
Specifications
Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with the binding buffer. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Tris, pH 7.4, 5 mM MgCl₂, 4 mM CaCl₂, 0.5 mM ascorbic acid, filtered and stored at 4°C
Radioligand: [³H]-5-Carboxamidotryptamine (Perkin Elmer NET1071)
Wash Buffer: same as the binding buffer.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 4-fold signal:background with ³H-labeled 5-CT at 30 nM
Physical form
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Storage and Stability
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service