MAB1562
Anti-Prion Protein Antibody, a.a. 109-112, clone 3F4
clone 3F4, Chemicon®, from mouse
Synonym(s):
PrP, CD230
About This Item
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F4, monoclonal
species reactivity
hamster, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PRNP(5621)
General description
Specificity
Immunogen
Application
Representative images from a previous lot. Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Brain
Immunohistochemistry (Kitamoto et al., 1987):
1:100-1:1,000 *See protocol below.
Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures:
a. formic acid for 10 minutes at room temperature (Kitamoto et al., 1987)
b. hydrolytic autoclaving (Kitamoto et al., 1991)
c. microwaving (BioGenex, San Ramon, CA)
Western Blot: (Kascsak, R.J., 1991; Kascsak, R.J., 1987):
1:10,000-1:100,000 dilution of a previous lot was used.
Immunoprecipitation: (Kascsak, R.J., 1991; Kascsak, R.J., 1987):
1:10-1:100 dilution of a previous lot was used.
ELISA: (Kascsak, R.J., 1991; Kascsak, R.J., 1987):
1:100,000 dilution of a previous lot was used.
Optimal working dilutions must be determined by end user.
Quality
Prion Protein (cat. # MAB1562) staining pattern/morphology in normal brain. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen predominantly as cell body staining of neurons.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Brain
Target description
Physical form
Legal Information
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service