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MAB1612

Sigma-Aldrich

Anti-Cytokeratin Epithelial Antibody, clone AE1

clone AE1, Chemicon®, from mouse

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Synonym(s):
Anti-CARD2, Anti-CK8, Anti-CYK8, Anti-K2C8, Anti-K8, Anti-KO
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AE1, monoclonal

species reactivity

mouse, chicken, human, rabbit, bovine, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KRT1(3848)
rat ... Krt19(360626)

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This Item
C5301C2931F3418
antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

AE1, monoclonal

clone

M20, monoclonal

clone

C-11, monoclonal

clone

C-11, monoclonal

species reactivity

mouse, chicken, human, rabbit, bovine, rat

species reactivity

feline, human, rabbit, canine, bovine

species reactivity

bovine, mouse, frog, human, kangaroo rat, rat

species reactivity

bovine, mouse, frog, human, kangaroo rat, rat

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunohistochemistry: suitable (paraffin)

technique(s)

immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using protease-digested sections of human or animal tissues, immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:400 using protease-digested, formalin-fixed, paraffin-embedded sections of human or animal tissues, western blot: suitable

technique(s)

direct immunofluorescence: 1:50 using PtK2 cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human placenta, western blot: suitable

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Specificity

Recognizes most of the acidic (Type I) keratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. MAB1612 produces heterogeneous staining within a given epithelium; the detailed staining pattern varies depending on the differentiated and/or growth state of individual cells.

Immunogen

Human epidermal keratin

Application

Immunohistochemistry on unfixed frozen sections and formalin-fixed paraffin embedded tissues. Antigen retrieval is recommended. HEIR or protease treatment can be used.

Western Blotting: AE1 recognizes LMW cytokeratins from the acidic subfamily including: Clone AE1 recognises the 56.5, 50, 50′, 48 and 40kD human cytokeratins of the acidic subfamily.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Cytokeratins
This Anti-Cytokeratin Epithelial Antibody, clone AE1 is validated for use in IH(P) for the detection of Cytokeratin Epithelial.

Linkage

Replaces: 04-588

Physical form

Format: Purified
Liquid in 0.05M Borate Buffered Saline, 0.15M NaCl, 0.09% Sodium Azide, pH8.2.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Marnie L Peterson et al.
Biochemistry, 45(7), 2387-2397 (2006-02-16)
Many exotoxins of Gram-positive bacteria, such as superantigens [staphylococcal enterotoxins, toxic shock syndrome toxin-1 (TSST-1), and streptococcal pyrogenic exotoxins] and anthrax toxin are bioterrorism agents that cause diseases by immunostimulation or cytotoxicity. Glycerol monolaurate (GML), a fatty acid monoester found
Tadashi Kitazawa et al.
Journal of oral science, 66(1), 15-19 (2023-11-27)
After tooth extraction, preservation of the alveolar ridge by socket grafting attenuates bone resorption. Runt-related transcription factor 2 (RUNX2) and SP7/Osterix (OSX) are transcription factors playing an important role in osteoblast differentiation. The purpose of this study was to evaluate
Marnie L Peterson et al.
Infection and immunity, 73(4), 2164-2174 (2005-03-24)
Despite knowledge of the effects of toxic shock syndrome (TSS) toxin 1 (TSST-1) on the adaptive immune system, little is known about stimulation of the innate immune system, particularly epithelial cells. This study investigated the interactions of TSS Staphylococcus aureus
Jagadeesh Janjanam et al.
Proteomics, 13(21), 3189-3204 (2013-09-14)
Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is
Tammy Ader et al.
Mechanisms of development, 123(2), 177-194 (2006-01-18)
Bile duct morphogenesis involves sequential induction of biliary specific gene expression, bilayer generation, cell proliferation, remodeling and apoptosis. HBC-3 cells are a model system to study differentiation of hepatoblasts along the hepatocytic or bile ductular lineage in vitro and in

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