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MAB3100

Sigma-Aldrich

Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2

clone 8A11.2, Chemicon®, from mouse

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Synonym(s):
Cx45, Gap Junction gamma-1 protein, Gap Junction alpha-7 protein, Cx-45, GJA7 protein, GJC1 protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8A11.2, monoclonal

species reactivity

dog (Wang, et al 2001), rabbit (Chaytor, et al 2005), chicken (Elenes, et al 2001), mouse, human, rat (Sorensen, et al 2008)

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

dog ... Gjc1(490936)
human ... GJC1(10052)
mouse ... Gjc1(14615)
rat ... Gjc1(266706)

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antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

8A11.2, monoclonal

clone

polyclonal

clone

P3C9, monoclonal

clone

5B9.2, monoclonal

species reactivity

dog (Wang, et al 2001), rabbit (Chaytor, et al 2005), chicken (Elenes, et al 2001), mouse, human, rat (Sorensen, et al 2008)

species reactivity

rat, human, mouse

species reactivity

rat, mouse

species reactivity

rat, rabbit, human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

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General description

Connexin 45 is member of a family of gap junction proteins known as Connexin gene family. It is expressed in a wide variety of cell types during development and adult life, particularly in cardiac and smooth muscle tissues as well as brain tissues. Connexin 45 forms part of a network of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. There a number of alternatively spliced transcript variants that all seen to encode the same protein isoform.

Specificity

Clone 8A11.2 is highly specific for Connexin 45 protein with no cross reactivity as analyzed by western blotting to any other connexin family member.
Others species not tested. Also confirmed to work on rat C6 cells.

Immunogen

Epitope: near C-terminus, cytoplasmic
The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.

Application

Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 8A11.2 is an antibody against Connexin 45 for use in IC, IH & WB.
Immunblotting (~46 kDa): 0.5 -1 µg/mL with ECL detection. Samples should be prepared with proteinase inhibitors and kept cold for best results. Alkali extraction methods can be used to enrich for connexin protein fractions. Briefly, 1 ml of NaHCO3 containing protease and phosphatase inhibitors and 22 µl of 1 M NaOH were added to frozen cell pellets. This cell suspension was sonicated for 30 s and incubated on ice for 50 min. Then, the homogenate was centrifuged for 30 min at 30,000 X g at 4°C. The supernatant was discarded, and the pellet was resuspended in sample buffer. {Elenes, S et al 2001}. 8% SDS_PAGE gels and PVDF membranes are suggested for greatest clarity.

Immunohistochemistry: fresh frozen, methanol fixed sections {Ujiie H et al (2003)}; 4% fixed frozen sections {Ikeda, Y et al 2007). 1-10 µg/mL with overnight incubations; 4% PFA fixed, paraffin-embedded sections {Gluhak-Heinrich, J et al 2007}, 1:50 with enzymatic detection.

Immunocytochemistry: 2% PFA fixed cells (15’, RT), permeabilized with a solution of 0.2% triton X-100/4% BSA {Sorensen et al 2008}.


Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Physical form

Format: Purified
Liquid at 1 mg/mL in 0.02M PB with 0.25M NaCl,pH=7.6 containing 0.1% sodium azide as a preservative.

Storage and Stability

Maintain at 2° to 8°C in undiluted aliquots for up to 12 months fromd date of receipt. Prevent multiple warming and coolings. Aliquot in tightly closed tubes to prevent loss.

Analysis Note

Control
Cardiac tissue, particularly sinoatrial tissues.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Xianming Lin et al.
American journal of physiology. Heart and circulatory physiology, 288(3), H1113-H1123 (2004-10-30)
The ventricular action potential was applied to paired neonatal murine ventricular myocytes in the dual whole cell configuration. During peak action potential voltages >100 mV, junctional conductance (g(j)) declined by 50%. This transjunctional voltage (V(j))-dependent inactivation exhibited two time constants
Eun Ju Choi et al.
Biomolecules, 10(10) (2020-10-03)
 Gap junctions (GJs) are intercellular channels that connect adjacent cells electrically and metabolically. The iodide-yellow fluorescent protein (I-YFP) gap junctional intercellular communication (GJIC) assay is a recently developed method with high sensitivity. HeLa cells have been widely used as GJ-deficient
Mahendra Kashyap et al.
American journal of clinical and experimental urology, 8(5), 163-176 (2020-11-26)
Expression of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels is reported in bladder, but the functional role remains unsettled. Here, we immunolocalized the HCN1 and HCN4 subtype in human bladder and investigated their functional significance. Bladder procured from ten organ donors was
Qiong Ke et al.
Scientific reports, 7(1), 458-458 (2017-03-30)
Somatic cells can be successfully reprogrammed into pluripotent stem cells by the ectopic expression of defined transcriptional factors. However, improved efficiency and better understanding the molecular mechanism underlying reprogramming are still required. In the present study, a scrape loading/dye transfer
John E Rash et al.
Journal of neurocytology, 34(3-5), 307-341 (2006-07-15)
Odorant/receptor binding and initial olfactory information processing occurs in olfactory receptor neurons (ORNs) within the olfactory epithelium. Subsequent information coding involves high-frequency spike synchronization of paired mitral/tufted cell dendrites within olfactory bulb (OB) glomeruli via positive feedback between glutamate receptors

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