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HAT Media Supplement (50×) Hybri-Max

γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

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Synonym(s):
HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

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H0137A5539O5003
vibrant-m

O5003

OPI Media Supplement

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioXtra

product line

BioXtra

product line

BioXtra

product line

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

sterility

γ-irradiated

sterility

γ-irradiated

sterility

γ-irradiated

sterility

γ-irradiated

Application

HAT Media Supplement (50×) Hybri-Max has been used:
  • in the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clones
  • in the generation of mAbs (monoclonal antibodies)
  • in the luciferase-based bioassay (LUC) assay

Reconstitution

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Repr. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges


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25G
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Journal of anatomy, 195 ( Pt 2), 161-171 (1999-10-21)
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This study was undertaken to develop a novel anti-citrullinated peptide antibody (ACPA) and to investigate its arthritogenicity in a collagen-induced arthritis (CIA) model. The novel ACPA, 12G1, was developed by injecting cyclic citrullinated antigen in mice and subsequently hybridizing the
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