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P7181

Sigma-Aldrich

Polyethylene glycol solution

Hybri-Max, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

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MDL number:
PubChem Substance ID:

grade

Hybri-Max

sterility

0.2 μm filtered

product line

BioReagent

form

solution

mol wt

average mol wt 1,450

concentration

50 % (w/v)

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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vibrant-m

P7181

Polyethylene glycol solution

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P1458

Polyethylene glycol solution

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vibrant-m

76293

Polyethylene glycol solution

Premium Grade
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, none detected, phosphatases, none detected, proteases, none detected

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

mol wt

average mol wt 1,450

mol wt

average mol wt 1,450

mol wt

average mol wt 8,000

mol wt

-

grade

Hybri-Max

grade

Hybri-Max

grade

-

grade

for molecular biology

General description

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Application

Polyethylene glycol solution has been used in the hybridization of the following:
  • irradiated camel fibroblast cells with A23 hamster cells
  • mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
  • rabbit splenocytes and cell line 240E-1

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Biochem/physiol Actions

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

Packaging

Packaged in sealed ampules under nitrogen.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

212.0 °F - closed cup

flash_point_c

100 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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