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P7306

Sigma-Aldrich

Polyethylene glycol/ dimethyl sulfoxide solution

Hybri-Max, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

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MDL number:
PubChem Substance ID:
NACRES:
NA.75

grade

Hybri-Max

Quality Level

sterility

0.2 μm filtered

product line

BioReagent

form

solution

mol wt

average mol wt 1,450

concentration

50 % (w/v)

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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This Item
P7181P1458764752
vibrant-m

P7181

Polyethylene glycol solution

vibrant-m

P1458

Polyethylene glycol solution

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

average mol wt 1,450

mol wt

average mol wt 1,450

mol wt

average mol wt 8,000

mol wt

PEG average Mn 5,000, PLGA Mn 55,000, average Mn 60,000 (total)

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

-

grade

Hybri-Max

grade

Hybri-Max

grade

-

grade

-

Application

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.


Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.

Packaging

Packaged in sealed ampules under nitrogen.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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