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HT Media Supplement (50×) Hybri-Max

lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma

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Synonym(s):
HT Supplement, Hypoxanthine-Thymidin (HT) media supplement

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

−20°C

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OPI Media Supplement

product line

BioXtra

product line

BioXtra

product line

BioXtra

product line

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.

Reconstitution

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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