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Sigma-Aldrich

Red Blood Cell Lysing Buffer Hybri-Max

liquid, sterile-filtered, suitable for hybridoma

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Synonym(s):
RBC Lysing Buffer
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

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This Item
H8889P7539A7422
vibrant-m

H8889

Histopaque®-1077 Hybri-Max

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable, single cell analysis: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

0.1 μm filtered

sterility

γ-irradiated

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

form

liquid

form

liquid

form

liquid

form

lyophilized powder

RBC lysing buffer, RBC solubilization buffer

density gradient, gradient centrifugation, lymphocyte purification reagent

Pen-Strep solution, Pen/Strep antibiotic solution, Pen Strep, tissue culture

Sigma-Aldrich A7422, AT Media Supplement (50×) Hybri-Maxγ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma, adenine-thymidine cocktail, AT media hybridoma selection, fusion media

Application

Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.

Biochem/physiol Actions

Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.

Components

Contains 8.3 g/L ammonium chloride in 0.01 M Tris-HCl buffer.

Other Notes

Note: This product is intended for the removal of red blood cells from mice. This product may not be appropriate for the lysis of red blood cells of other animals. The suitability of the product in any application other than mouse splenocytes must be determined by the researcher.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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