MilliporeSigma
All Photos(1)

Documents

O5003

Sigma-Aldrich

OPI Media Supplement

Hybri-Max, γ-irradiated, powder, suitable for hybridoma

Sign Into View Organizational & Contract Pricing

Synonym(s):
OPI Media for cell culture
NACRES:
NA.75

grade

Hybri-Max

Quality Level

sterility

γ-irradiated

form

powder

concentration

18-40 mg/vial

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
H0262A7422A9666
vibrant-m

O5003

OPI Media Supplement

vibrant-m

A9666

Azaserine-Hypoxanthine 50x

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

sterility

γ-irradiated

sterility

γ-irradiated

sterility

γ-irradiated

sterility

γ-irradiated

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

grade

Hybri-Max

grade

-

grade

-

grade

Hybri-Max

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

Application

OPI Media Supplement has been used to grow hybridoma cell lines from XenoMouse strains and BALB/c female mice.

Other Notes

Formulated with: 0.15 g oxaloacetate, 0.05 g pyruvate, and 0.0082 g insulin.

Reconstitution

Reconstitute contents of vial with 10 mL sterile water. Do not use cell culture medium for initial reconstitution. Each vial sufficient to prepare 1 liter of medium. Final working concentration: 1 mM oxaloacetate, 0.45 mM pyruvate, 0.2 U/ml insulin.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1 - Skin Sens. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 5

1 of 5

SAFC

SAFC

H4281

EX-CELL® Hybridoma Medium

SAFC

SAFC

N1140

NCTC 109 Medium

Nutridoma™-SP suitable for hybridoma, solution, pkg of 100 mL (100x)

Roche

11011375001

Nutridoma-SP

Aminopterin Hybri-Max™, 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

Sigma-Aldrich

A5159

Aminopterin

Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
Gabriela A Canziani et al.
Analytical biochemistry, 325(2), 301-307 (2004-01-31)
Experimental and data analysis protocols were developed to screen antibodies from hybridoma culture supernatants using Biacore surface plasmon resonance biosensor platforms. The screening methods involved capturing antibodies from crude supernatants using Fc-specific antibody surfaces and monitoring antigen binding at a
Jun Yang et al.
Clinical biochemistry, 48(13-14), 843-848 (2015-05-31)
Neurogranin (NRGN) is a small neuronal protein that plays an important role in synaptic signaling by regulating calmodulin (CaM) availability. In this study, we developed an ELISA to measure NRGN quantitatively in serum samples from a cohort of acute traumatic
Andrew W Drake et al.
Analytical biochemistry, 328(1), 35-43 (2004-04-15)
Two biophysical methods, Biacore and KinExA, were used to kinetically and thermodynamically characterize high-affinity antigen/antibody complexes. Three to five independent experiments were performed on each platform with three different antigen/antibody complexes possessing nanomolar to picomolar equilibrium dissociation constants. By monitoring
Tzu-Ling Sung et al.
PLoS pathogens, 5(1), e1000263-e1000263 (2009-01-17)
Cyclin T1 is a regulatory subunit of a general RNA polymerase II elongation factor known as P-TEFb. Cyclin T1 is also required for Tat transactivation of HIV-1 LTR-directed gene expression. Translation of Cyclin T1 mRNA has been shown to be

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service