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P4780

Sigma-Aldrich

TWEEN® 80

suitable for cell culture, suitable for insect cell culture, viscous liquid

Synonym(s):
POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80
CAS Number:
MDL number:
NACRES:
NA.25

description

non-ionic

Quality Level

form

viscous liquid

mol wt

average mol wt ~1310

composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

CMC

0.012 mM

density

1.06 g/mL at 25 °C (lit.)
1.07 g/cm3 at 25 °C (lit.)

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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P80748.22187655207
TWEEN® 80 suitable for cell culture, suitable for insect cell culture, viscous liquid

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P4780

TWEEN® 80

TWEEN® 80 BioXtra, viscous liquid

Sigma-Aldrich

P8074

TWEEN® 80

TWEEN® 80 for synthesis

Sigma-Aldrich

8.22187

TWEEN® 80

TWEEN® 80 PROTEIN GRADE Detergent, 10% Solution, Sterile-Filtered. Non-ionic detergent used for immunoprecipitation and for the solubilization of membrane-bound proteins. Supplied as a 10% (W/W) solution of PROTEIN GRADE TWEEN 80 detergent. Conductivity:≤50 µmhos.

Millipore

655207

TWEEN® 80

form

viscous liquid

form

viscous liquid

form

liquid

form

liquid

mol wt

average mol wt ~1310

mol wt

micellar avg mol wt 79,000, average mol wt 1310

mol wt

-

mol wt

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable

technique(s)

-

technique(s)

-

CMC

0.012 mM

CMC

0.012 mM (20-25°C)

CMC

-

CMC

12 μM (HLB number 15)

density

1.06 g/mL at 25 °C (lit.), 1.07 g/cm3 at 25 °C (lit.)

density

1.06 g/mL at 25 °C

density

1.07 g/cm3 at 25 °C

density

-

General description

TWEEN 80 is a polyethylene sorbitol ester and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
TWEEN® 80 has been used:
  • in Listeria monocytogenes enumeration using filtration method
  • as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion
  • as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Polysorbate 80 tested according to Ph. Eur.

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Polysorbate 80

TWEEN® 80 PROTEIN GRADE Detergent, 10% Solution, Sterile-Filtered. Non-ionic detergent used for immunoprecipitation and for the solubilization of membrane-bound proteins. Supplied as a 10% (W/W) solution of PROTEIN GRADE TWEEN 80 detergent. Conductivity:≤50 µmhos.

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TWEEN® 80

Span® 80 nonionic surfactant

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S6760

Span® 80

TWEEN® 80 (Polysorbate) EMPROVE® ESSENTIAL, Ph. Eur., JP, NF

SAFC

8.17061

TWEEN® 80 (Polysorbate)

Polysorbate 80 FG

Sigma-Aldrich

W291706

Polysorbate 80

TWEEN® 20 viscous liquid, suitable for cell culture

Sigma-Aldrich

P2287

TWEEN® 20

Polysorbate 80 United States Pharmacopeia (USP) Reference Standard

USP

1547969

Polysorbate 80

Low-fat Gouda cheese made from bovine milk-olive oil emulsion: physicochemical and sensory attributes
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Journal of Food Science and Technology, 52(10), 6749-6755 (2015)
A contribution to the improvement of Listeria monocytogenes enumeration in cold-smoked salmon
Besse NG, e al.
International Journal of Food Microbiology, 91(2), 119-127 (2004)
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