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MABF3201

Sigma-Aldrich

Anti-MART-1/Melan-A Antibody, clone A103

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Synonym(s):
Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, Protein Melan-A

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

A103, monoclonal

mol wt

calculated mol wt 13.16 kDa
observed mol wt ~16 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 100 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

Unknown

Protein ID accession no.

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... MLANA(2315)

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species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

mouse, canine, human

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:1000-1:2500

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

Gene Information

human ... MLANA(2315)

Gene Information

human ... MLANA(2315)

Gene Information

human ... MLANA(2315)

Gene Information

human ... MCAM(4162)

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General description

Melanoma antigen recognized by T-cells 1 (UniProt: Q16655; also known as MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A) is encoded by the MLANA (also known as MART1) gene (Gene ID: 2315) in human. MART-1, a melanosome-specific protein, is a single-pass type III membrane protein with a transmembrane domain (aa 27-47) and a cytoplasmic domain (aa 48-118). Its expression is restricted to melanocytes, melanoma, and retina and is absent in other tissues and tumors. It is highly enriched in early melanosomes (Stage I and/or II melanosomes) indicating that its role in early melanogenesis. It is reported to stabilize both GPCR143/OA-1 and plays a role in the expression, stability, trafficking, and processing of pre-melanosome protein (PMEL) to ensure the formation of a stage 2 melanosome. It is mainly found in the endoplasmic reticulum (ER) and Golgi but is also detected in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation, which allows melanoma cells to be effectively recognized by specific cytotoxic T-cells. MART-1 may be a useful marker in the diagnosis of melanocytic lesions and in the differential diagnosis of malignant melanoma. (Ref.: Hoashi, T., et al. (2005). J. Biol. Chem. 280(14); 14006-14016; Chen, YT., et al. (1996). Proc. Natl. Acad. Sci. USA. 93(12); 5915-5919).

Specificity

Clone A103 is a mouse monoclonal antibody that detects MART-1.

Immunogen

Full-length, recombinant, human MART-1 produced in E.coli.

Application

Quality Control Applications

Evaluated by Western Blotting in SK-MEL-28 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MART-1 in SK-MEL-28 cell lysate.

Tested applications

Western Blotting Analysis: A representative lot detected MART-1 in Western Blotting applicaiton. (Chen, Y.T., et al. (1996). Proc Natl Acad Sci USA. 93(12); 5915-9).

Immunohistochemistry Applications: A representative lot detected MART-1/Melan-A in Immunohistochemistry applications (Chen, Y.T., et al. (1996). Proc Natl Acad Sci USA. 93(12);5915-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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