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M6570

Sigma-Aldrich

Anti-Melan-A antibody, Mouse monoclonal

clone A103, purified from hybridoma cell culture

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Synonym(s):
Anti-MART1
MDL number:

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

A103, monoclonal

form

buffered aqueous solution

mol wt

antigen ~22 kDa

species reactivity

human, canine

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of SKMEL19 cells ()

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MLANA(2315)

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This Item
SAB4500949MABF3201HPA048662
species reactivity

human, canine

species reactivity

human

species reactivity

human

species reactivity

human

clone

A103, monoclonal

clone

polyclonal

clone

A103, monoclonal

clone

polyclonal

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

Gene Information

human ... MLANA(2315)

Gene Information

human ... MLANA(2315)

Gene Information

human ... MLANA(2315)

Gene Information

human ... MLANA(2315)

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General description

Melan-A is also known as MART-1 (melanoma antigen recognized by T-cells 1). It is a transmembrane protein which is hydrophobic in nature. Melan-A is expressed in melanocytes and melanoma tumors.
Monoclonal Anti-Melan-A (mouse IgG1 isotype) is derived from the hybridoma A103 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with a human recombinant Melan-A protein. Melan-A (also known as MART1) is a melanoma antigen recognized by T cells. The protein is expressed mainly in cells and tumors of melanocytic lineage and thus belongs to the melanocyte differentiation markers.

Immunogen

recombinant human Melan-A protein.

Application

Anti-Melan-A antibody, Mouse monoclonal has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry
  • immunoprecipitation
  • immunohistochemistry

Biochem/physiol Actions

Melan-A/MART1 may have a role in pigmentation since its expression pattern and sub cellular distribution is similar to other melanosomal proteins and correlates with melanin content. Melan-A is important for Pmel17 activity, forms a complex with it, and affects its expression, stability, trafficking, and processing that is required for melanosome structure and maturation. Thus, Melan-A is important for Pmel17 function and therefore it is important for mammalian pigmentation.

Target description

Melan-A is a melanoma antigen recognized by T cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Enayat Attwa et al.
International journal of dermatology, 54(2), 235-244 (2014-10-01)
Melasma is a common pigmentary disorder that remains resistant to available therapies. The aim of the present study was to evaluate the efficacy of erbium:YAG lasers in the treatment of refractory melasma and investigate the histopathological and ultrastructural changes between
Cytokines and Biologic Modifiers
Immunology for Pharmacy, 181-188 (2012)
Lin Hou et al.
Theranostics, 8(14), 3781-3796 (2018-08-08)
Rationale: Tumor-associated fibroblasts (TAFs) play a critical role in the suppressive immune tumor microenvironment (TME), compromising the efficacy of immunotherapy. To overcome this therapeutic hurdle, we developed a nanoemulsion (NE) formulation to deliver fraxinellone (Frax), an anti-fibrotic medicine, to TAFs
Aleksandra Simiczyjew et al.
Journal of cellular and molecular medicine, 27(19), 2995-3008 (2023-09-08)
Mucosal melanoma (MM) is a very rare and aggressive type of cancer for which immunotherapy or targeted therapy such as BRAF/MEK inhibitors, used in cutaneous melanoma, usually fail. Due to our earlier experience showing the high effectiveness of epidermal growth
MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes.
Hoashi T, et al.
The Journal of Biological Chemistry, 280(14), 14006-14016 (2005)

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