MilliporeSigma
All Photos(1)

Documents

MABT219

Sigma-Aldrich

Anti-Actin Antibody, clone JLA20

clone JLA20, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Actin, alpha skeletal muscle, Alpha-actin-1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

JLA20, monoclonal

species reactivity

human

species reactivity (predicted by homology)

chicken (immunogen homology)

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ACTA1(58)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABT826MAB1501RABT1487
Anti-Actin Antibody, clone JLA20 clone JLA20, from mouse

MABT219

Anti-Actin Antibody, clone JLA20

Anti-Actin Antibody, clone 2G2 clone 2G2, from mouse

MABT826

Anti-Actin Antibody, clone 2G2

Anti-Actin Antibody, clone C4 clone C4, Chemicon®, from mouse

MAB1501R

Anti-Actin Antibody, clone C4

Gene Information

human ... ACTA1(58)

Gene Information

human ... ACTA1(58)

Gene Information

human ... ACTA1(58)

Gene Information

human ... ACTA1(58)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

affinity isolated antibody

clone

JLA20, monoclonal

clone

2G2, monoclonal

clone

C4, monoclonal

clone

polyclonal

UniProt accession no.

P68139

UniProt accession no.

P68135

UniProt accession no.

P68133

UniProt accession no.

P62736

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Actin is an abundantly expressed in eukaryotic cells and it is the core component of the cytoskeleton. It exists in three isoforms: alpha actin, which is found in muscle tissue and forms fibrous actin that facilitates muscle contractility; and beta and gamma actin which are found in non-muscle cells and facilitate cell motility. Actin exists initially as globular actin (G-actin) which is converted to filamentous actin (F-actin) upon assembly. The formation of F-actin is a complex multistep process that is tightly regulated by signaling from the actin-binding proteins (ABPs) and Rho family GTPases. Filamentous actin is crosslinked with other actin polymers to form a complex cytoskeletal network which is required for exo- and phagocytosis, vesicular trafficking, cell migration, and gene regulation, among other cellular processes.

Application

Detect Actin using this Anti-Actin Antibody, clone JLA20 validated for use in Western Blotting, Immunofluorescence.
Immunofluorescence Analysis: A representative lot was used by an independent laboratory in NRK cells. (Cao, L.G., et al. (1993). The Journal of Cell Biology. 123(1):173-181.)

Western Blot Analysis: A representative lot was used by an independent laboratory in Me4405 melanoma cell lysate. (Thomas, W.D., et al. (2000). Journal of Immunology. 165:5612–5620.)

Quality

Evaluated by Western Blot in SK-BR3 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Actin on 10 µg of SK-BR3 cell lysate.

Target description

~42 kDa observed

Linkage

Replaces: 04-1040

Physical form

Format: Purified

Analysis Note

Control
SK-BR3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Carwyn Davies et al.
The Journal of biological chemistry, 286(43), 37639-37650 (2011-09-10)
The p53 protein is a primary mediator of cellular apoptosis and growth arrest after exposure to DNA-damaging agents. Previous work has shown that the majority of childhood acute lymphoblastic leukemia (ALL) cases express a wild type p53 gene, although the
Jennifer Huggins et al.
Blood, 109(4), 1611-1619 (2006-10-13)
Unmethylated CpG DNA activation of naive CD27- B cells has been reported to require B-cell-receptor (BCR) cross-linking. We describe a culture system using CpG DNA with sequential steps for T-cell-independent activation of naive CD19+CD27- human peripheral blood B cells that
Stefania Bassanini et al.
Neurobiology of disease, 26(2), 481-495 (2007-04-03)
One of the most common causes of neurological disabilities are malformations of cortical development (MCD). A useful animal model of MCD consists of prenatal exposure to methylazoxymethanol (MAM), resulting in a postnatal phenotype characterized by cytological aberrations reminiscent of human
Erik J Tokar et al.
Journal of the National Cancer Institute, 102(9), 638-649 (2010-03-27)
Arsenic is a carcinogen that targets the urogenital system, including the prostate. Although the mechanisms for arsenic-induced carcinogenesis are undefined, arsenic drives overaccumulation of stem cells and cancer stem cells (CSCs) in vivo and in vitro, indicating that these cells
Keisuke Taniuchi et al.
Cancer medicine, 7(11), 5733-5751 (2018-10-26)
WAVE2 is a member of the WASP/WAVE family of actin cytoskeletal regulatory proteins; unfortunately, little is known about its function in pancreatic cancers. In this study, we report the role of WAVE2 in the motility and invasiveness of pancreatic cancer

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service