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MABT892

Sigma-Aldrich

Anti-SUN1 Antibody, clone 12.10F

clone 12.10F, from mouse

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Synonym(s):
SUN domain-containing protein 1, Protein unc-84 homolog A, Sad1/unc-84 protein-like 1
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

12.10F, monoclonal

species reactivity

mouse, rat

should not react with

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

mouse ... Sun1(77053)

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This Item
ABT273MABT880MABT843
Anti-SUN1 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABT273

Anti-SUN1 Antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

clone

12.10F, monoclonal

clone

polyclonal

clone

3.1E, monoclonal

clone

7A12, monoclonal

species reactivity

mouse, rat

species reactivity

rat, human

species reactivity

human, rat, mouse

species reactivity

rat, human, mouse, rabbit

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

isotype

IgG2aκ

isotype

-

isotype

IgG1κ

isotype

IgG1κ

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General description

SUN domain-containing protein 1 (UniProt Q9D666; also known as Protein unc-84 homolog A, Sad1/unc-84 protein-like 1) is encoded by the Sun1 (also known as Unc84a) gene (Gene ID 77053) in murine species. SUN1 is a component of the SUN-protein-containing complexes known as LINC (linker of nucleoskeleton and cytoskeleton), which link the nucleoskeleton and cytoskeleton structures together in a scaffold like fashion. SUN1 is required for interkinetic nuclear migration and nucleokinesis as well as nucleus-centrosome coupling during radial neuronal migration and glial migration in the cortex. SUN1 also is important during meiosis and is required for proper gametogenesis. It also participates in anchoring chromosome movement in the prophase of meiosis and in selective gene expression of coding and non-coding RNAs required for gametogenesis. Additionally, it has a vital role in the distribution of nuclear pore complexes on the nuclear membrane. Interestingly, LINC complexes are also critical for vital functions such as hearing.

Specificity

Clone 12.10F was raised against a fusion construct composed of murine SUN1 exon 6-, 8-, and 10-encoded regions that are present in murine spliced isoforms 1, 3, and 4 reported by UniProt (Q9D666). The epitope location among these three regions has not been mapped and reactivity toward murine spliced isoform 3 is expected only if the epitope resides within exon 1- or 4-encoded region.

Immunogen

GST-tagged recombinant fusion protein composed of murine SUN1 regions encoded by exons 6, 8, and 10.

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected SUN1 nuclear immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized C2C12 murine myoblasts.

Immunocytochemistry Analysis: A representative lot immunostained nucleus of C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore).

Western Blotting Analysis: A representative lot detected greatly reduced SUN1 isoforms target bands in lysate from SUN1 siRNA-treated C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore).
Research Category
Cell Structure
This mouse monoclonal Anti-SUN1 Antibody, clone 12.10F, Cat. No. MABT892 is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN1.

Quality

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN1 in 10 µg of C2C12 murine myoblast lysate.

Target description

~102 kDa oberved. 102.0/88.20/97.92/94.51 kDa (mouse isoform 1/2/3/4) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified.
Purified mous IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Mark A Smith et al.
Heliyon, 8(12), e12147-e12147 (2023-01-10)
Formation of robust actomyosin stress fibers (SF) in response to cell stretch plays a key role in the transfer of information from the cytoplasm into the nucleus. Actin/LINC/Lamin (ALL) nuclear lines provide mechanical linkage between the actin cytoskeleton and the
Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope.
Sharma, et al.
Life science alliance, 6 (2023)

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