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11333062910

Roche

Anti-Digoxigenin

from mouse IgG1κ (clone 1.71.256)

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Synonym(s):
anti-digoxigenin, digoxigenin

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

clone 1.71.256, monoclonal

form

lyophilized

packaging

pkg of 100 μg

manufacturer/tradename

Roche

technique(s)

ELISA: 2-4 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.5-2 μg/mL
western blot: 0.5-2 μg/mL

isotype

IgG1κ

storage temp.

2-8°C

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Anti-Digoxigenin from mouse IgG1κ (clone 1.71.256)

11333062910

Anti-Digoxigenin

Anti-Digoxigenin from sheep

11333089001

Anti-Digoxigenin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

rhodamine conjugate

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

clone

clone 1.71.256, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

technique(s)

ELISA: 2-4 μg/mL, immunohistochemistry: 0.5-2 μg/mL, immunocytochemistry: 0.5-2 μg/mL, western blot: 0.5-2 μg/mL

technique(s)

-

technique(s)

ELISA: 2-4 μg/mL, immunohistochemistry: 0.5-1 μg/mL, western blot: 0.5-2 μg/mL

technique(s)

-

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized (clear, red solution after reconstitution)

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General description

Contents: Lyophilizate
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.

Specificity

The monoclonal antibody reacts with free and bound digoxigenin.

Application

Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot
  • Immunofluorescence staining
  • FISH (fluorescent in situ hybridization)

Biochem/physiol Actions

In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.

Features and Benefits

  • Specific to free and bound digoxigenin
  • The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.

Preparation Note

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications
phosphate buffered saline, pH 7.4

Reconstitution

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.

Storage and Stability

The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.

Analysis Note

There are no cross reactivities known.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+-ATPase by N-Hydroxysuccinimidyl Derivatives of Digoxigenin
<BIG>Antolovic R, et al.</BIG>
European Journal of Biochemistry, 227, 61-67 (1995)
Carla Winterling et al.
RNA biology, 11(1), 66-75 (2014-01-21)
A growing body of evidence suggests the non-protein coding human genome is of vital importance for human cell function. Besides small RNAs, the diverse class of long non-coding RNAs (lncRNAs) recently came into focus. However, their relevance for infection, a
Ilyas Chachoua et al.
Nature communications, 13(1), 204-204 (2022-01-13)
Abnormal WNT signaling increases MYC expression in colon cancer cells in part via oncogenic super-enhancer-(OSE)-mediated gating of the active MYC to the nuclear pore in a poorly understood process. We show here that the principal tenet of the WNT-regulated MYC
Xingqi Chen et al.
BioTechniques, 56(3), 117-118 (2014-03-20)
Current techniques for analyzing chromatin structures are hampered by either poor resolution at the individual cell level or the need for a large number of cells to obtain higher resolution. This is a major problem as it hampers our understanding
Kristiina Joensuu et al.
Breast cancer : basic and clinical research, 7, 23-34 (2013-03-22)
Breast cancer can recur even decades after the primary therapy. Markers are needed to predict cancer progression and the risk of late recurrence. The estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (HER2), proliferation marker Ki-67, and

Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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