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F3523

Sigma-Aldrich

Monoclonal Anti-Digoxin–FITC antibody produced in mouse

clone DI-22, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Monoclonal Anti-Digoxin
NACRES:
NA.46

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DI-22, monoclonal

form

buffered aqueous solution

storage condition

protect from light

extent of labeling

3-8 mol fluorochrome per mol protein

technique(s)

dot immunobinding: 1:16 using digoxigenin-labeled biomolecule

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
B0287A1054F4891
conjugate

FITC conjugate

conjugate

biotin conjugate

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

DI-22, monoclonal

clone

FL-D6, monoclonal

clone

DI-22, monoclonal

clone

GT-34, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

dot immunobinding: 1:16 using digoxigenin-labeled biomolecule

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil

technique(s)

direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate, direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate, dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)

technique(s)

dot immunobinding: 1:32, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, particle immunofluorescence: 1:32

General description

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Immunogen

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin-FITC antibody produced in mouse has been used in immuno fluorescence in situ hybridization (FISH) and FISH.

Biochem/physiol Actions

Monoclonal Anti-Digoxin- FITC may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides. Labeled compounds and corresponding conjugated antibodies can be used for the detection of viruses and bacterial infections in human diagnostics, oncogenes as tumor markers, histocompatibility antigens in transplantation analytics causative research (e.g., in autoimmune diseases), characterization of lymphoid cell subpopulations (e.g., during treatment of lymphomas), determination of genetic defects or genetic defect predispositions (e.g., Alzheimer′s disease), and nucleic acid diagnostics.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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