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11412272001

Roche

Transforming Growth Factor-β1, human (hTGF-β1)

recombinant (CHO cells)

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Synonym(s):
hTGF

biological source

human

Quality Level

recombinant

expressed in CHO cells

Assay

>95% (SDS-PAGE)

form

solution

potency

<0.1 ng/mL EC50

mol wt

25,000 Da

packaging

pkg of 1 mL (1 μg)

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

impurities

<0.1 EU/μgtested (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TGFB1(7040)

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This Item
T7039H8541H8666
recombinant

expressed in CHO cells

recombinant

expressed in CHO cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

assay

>95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

solution

form

powder

form

lyophilized powder

form

lyophilized powder

potency

<0.1 ng/mL EC50

potency

≤0.05 ng/mL ED50

potency

≤0.5 ng/mL EC50

potency

≤0.5 ng/mL EC50

mol wt

25,000 Da

mol wt

protein 25 kDa

mol wt

dimer 25 kDa (non-glycosylated)

mol wt

dimer 25 kDa (non-glycosylated)

General description

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.

Specificity

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC50: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.

Application

Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.

Biochem/physiol Actions

Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.
Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.

Quality

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)

Sequence

Chain Length 2 x 112 AA
Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

Unit Definition

EC50 definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.

Preparation Note

Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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