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H8666

Sigma-Aldrich

Transforming Growth Factor-β2 human

TGF-β2, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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Synonym(s):
TGF-β2
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤0.5 ng/mL EC50

quality

endotoxin tested

mol wt

dimer 25 kDa (non-glycosylated)

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TGFB2(7042)

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This Item
T7039T2815H8541
recombinant

expressed in HEK 293 cells

recombinant

expressed in CHO cells

recombinant

expressed in NSO cells

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥97% (electrophoresis)

assay

≥95% (SDS-PAGE)

form

lyophilized powder

form

powder

form

powder

form

lyophilized powder

potency

≤0.5 ng/mL EC50

potency

≤0.05 ng/mL ED50

potency

0.04 ng/mL ED50

potency

≤0.5 ng/mL EC50

mol wt

dimer 25 kDa (non-glycosylated)

mol wt

protein 25 kDa

mol wt

25 kDa

mol wt

dimer 25 kDa (non-glycosylated)

General description

Transforming growth factor-β2 (TGF-β2) belongs to the TGF-β superfamily. All threes isoforms (TGF-β1, TGF-β2 and TGF-β3) are known to share similar structure and function. The TGFB2 gene is mapped to human chromosome 1q41 and is localized to extracellular matrix. The active form of TGF-β precursor is produced upon proteolytic cleavage. TGF-β1 and TGF-β2 have 70% amino acid sequence identity.

Biochem/physiol Actions

TGF-β2 is important for immune homeostasis by balancing lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is crucial in cell growth, differentiation, and survival. TGF-β2 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-β is associated with the process of tumor development, progression and metastasis. TGF-β2 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils. TGF-β is also associated with inflammation and wound healing. The TGF-β gene is overexpressed in glioma tumors and also in colon cancer, gastric cancer and cervical lesions.
Transforming growth factor-β2 (TGF-β2), like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

Preparation Note

HumanKine TGF-β2 is expressed in human HEK 293 cells as a mature, non-glycosylated, disulfide-linked homodimer with a predicted molecular mass of approx. 25 kDa.

Analysis Note

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Evaluation of zinc finger E-box binding homeobox 1 and transforming growth factor-β2 expression in bladder cancer tissue in comparison with healthy adjacent tissue.
Mahdavinezhad A, et al.
Investigative and Clinical Urology, 58(2), 140-145 (2017)
A Signaling Pathway Involving TGF-β2 and Snail in Hair Follicle Morphogenesis
Jamora C, et al.
PLoS Biology, 3.1 (2004)
Loss-of-function mutations in TGFB2 cause a syndromic presentation of thoracic aortic aneurysm
Lindsay ME, et al.
Nature Genetics, 44(8), 922?927-922?927 (2012)
Sunder Sims-Lucas et al.
Differentiation; research in biological diversity, 79(4-5), 272-284 (2010-02-19)
Many members of the transforming growth factor-beta (TGF-beta) superfamily have been shown to be important regulators of metanephric development. In this study, we characterized the effect of TGF-beta2 on metanephric development. Rat and mouse metanephroi cultured in the presence of
Curcumin suppresses doxorubicin-induced epithelial?mesenchymal transition via the inhibition of TGF-β and PI3K/AKT signaling pathways in triple-negative breast cancer cells.
Chen WC, et al.
Journal of Agricultural and Food Chemistry, 61.48, 11817-11824 (2013)

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