AGRMP-RO
Roche
Agarose MP
powder, pkg of 100 g (11388983001), pkg of 500 g (11388991001)
Synonym(s):
agarose
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About This Item
UNSPSC Code:
12352200
Recommended Products
biological source
algae (seaweed)
description
multipurpose agarose
form
powder
packaging
pkg of 100 g (11388983001)
pkg of 500 g (11388991001)
manufacturer/tradename
Roche
impurities
0.090 sulphate
color
white
mp
38 °C
solubility
water: 10 g/L at 80 °C
storage temp.
15-25°C
20-25°C
General description
Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.
Application
Agarose MP, a multi purpose agarose, is developed for analytical and preparative electrophoresis of nucleic acids and separation of high molecular weight DNA via pulse field gel electrophoresis (PFGE).It is also used for preparative electrophoresis and quantitative recovery of biologically active DNA. Agarose MP has also been used along with other supplements to store embryos in different developmental stages.
Features and Benefits
- High Gel Strength: Agarose MP gels exhibit great strength, even at low concentrations. This ensures sturdy gels for robust performance in electrophoresis.
- Versatile Applications: Agarose MP aids in the analysis of various samples, including high molecular weight molecules.
- Rapid Migration without restriction: Even at low concentrations agarose MP gels facilitate the rapid migration of large DNA molecules without significant restriction, resulting in efficient separation.
- Pulsed-Field Gel Electrophoresis (PFGE) Compatibility: Agarose MP is ideal for PFGE as it enables short separation times for large DNA molecules while maintaining the high resolution required for effective analysis.
Quality
Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.
Other Notes
Agarose MP can produce high strength gels (e.g., the gel strength of a 1% agarose MP gel is ?1800 g/cm2). Even gels that have low concentrations of agarose MP are strong enough to be used for electrophoresis. The agarose matrix of such low concentration gels allows high molecular weight molecules to migrate rapidly without significant restriction.
Agarose MP is particularly well suited for pulsed-field gel electrophoresis, because it allows short separation times for large DNA molecules, while maintaining the high resolution required for effective analysis.
For life science research only. Not for use in diagnostic procedures.
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Pei Yun Lee et al.
Journal of visualized experiments : JoVE, 62(62), 3923-3923 (2012-05-02)
Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose)
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